Test Price
350 AED✅ Home Collection Available
Sequencing Library QC‑qPCR (NGS Library QC) in UAE | 350 AED | DHA Licensed
Executive Summary & Core Metrics
Core Metrics & Guarantees
- Diagnostic Accuracy 99.9%: ISO-accredited qPCR-based library quantification with CV below 3%, ensuring reliable NGS results and minimal re-run risk.
- Premium Logistics: VIP Mobile Phlebotomy & Temperature-Controlled Cold-Chain Home Collection available daily from 8 AM to 11 PM.
- Post-Test Clinical Guidance: Telephonic interpretation support by DHA-licensed specialists for all NGS library QC results.
- Insurance Verification: Direct billing pre-approval via WhatsApp at +971 54 548 8731 – instant confirmation before sample collection.
Clinical Alignment with UAE Standards
Federal Decree-Law No. 45 of 2021 (PDPL) • Federal Law No. 2 of 2019 (ICT in Health) • Federal Decree-Law No. 4 of 2016 (Medical Liability)
DHA Facility License No. 1143 • DNA Labs UAE
Test Overview & Methodology
Sequencing Library QC‑qPCR is the gold-standard quantitative PCR assay used to validate DNA library concentration and fragment size before Next-Generation Sequencing (NGS). It ensures only high-quality libraries with optimal molarity are loaded onto a sequencer, preventing run failures and guaranteeing reliable genomic data.
This method uses real-time PCR with SYBR Green or probe-based detection to measure amplifiable library molecules with sensitivity down to 0.2 nM. Unlike fluorometric methods, qPCR specifically quantifies fragments that carry the correct adapter sequences and are therefore suitable for cluster generation on the flow cell.
| Feature | Our Test (qPCR) | Closest Alternative (Fluorometry) |
|---|---|---|
| Precision | CV < 3%, detects < 0.2 nM | CV ~10%, limited to ng/µL |
| Method | Real-Time qPCR (SYBR Green or Probe) | Fluorometric dye binding (Qubit) |
| Turnaround Time | 1 week (includes library preparation and QC) | Same day (no NGS readiness confirmation) |
* Fluorescence alone cannot distinguish amplifiable library fragments from free primers or adapter dimers.
Methodology Detail
The assay employs a standard curve of known molarity to interpolate the concentration of each library. Adapter-ligated fragments are amplified in triplicate, and the resulting Cq values are converted to nanomolar concentration using the curve equation. This process discriminates against non-amplifiable material, providing a true measure of sequencing-ready molecules.
Physician Insight & Safety Protocols
“As a Consultant Medical Genetics, I consider sequencing library QC‑qPCR an essential gatekeeping step before any NGS run. Without precise quantification of amplifiable molecules, the risk of failed sequencing or biased variant calling increases substantially. This assay ensures that every library loaded on the sequencer meets the stringent quality thresholds required for accurate genomic interpretation, whether for rare disease diagnosis or oncology panel testing.”
— Lina Osama Zaki Quteineh, Consultant Medical Genetics • DHA Registration ID: 9294403
Clinical Advisory
This test is a laboratory quality control procedure for NGS library preparation and does not replace a clinical diagnostic evaluation or genetic counselling. Always consult your physician before making any decisions regarding your health or treatment plan.
Exclusion Criteria & Safety Red Flags
- Insufficient or degraded DNA: Sample may be rejected if concentration is below 50 ng/µL or if severe fragmentation is detected (Bioanalyzer RIN < 6).
- Home blood collection exclusions: Active bleeding disorder, INR > 3.0, pregnancy with haemodynamic instability, or severe anaemia (Hb < 8 g/dL).
- Emergency alert: If unexpected loss of consciousness, chest pain, or severe haemorrhage occurs after sample collection, call 998 immediately.
Patient FAQ & Clinical Guidance
1. What exactly does Sequencing Library QC‑qPCR measure, and why is it necessary before genetic testing?
It quantifies the exact amount of amplifiable DNA library molecules by amplifying adapter-ligated fragments, ensuring the sequencer receives the optimal concentration to generate accurate genetic reads without costly re-runs. This directly impacts the reliability of downstream variant calling and copy number analysis.
2. How does your qPCR library QC differ from standard fluorometric quantification methods used in other laboratories?
Our laboratory uses ISO 9001:2015-calibrated qPCR systems with library-specific standard curves, providing ten-fold higher quantitative accuracy than standalone fluorometry. This method specifically detects only adapter-ligated, amplifiable fragments, dramatically reducing the risk of sequencing failure due to overestimation of library concentration.
3. Can I provide a blood sample at home instead of visiting the lab, and how long will the complete process take?
Yes, our DHA-licensed mobile phlebotomist can collect a whole blood sample from your residence between 8 AM and 11 PM using a temperature-controlled cold chain. Once the sample is received at our laboratory, the entire workflow from DNA extraction through library preparation, qPCR QC, and reporting is completed within one week.
4. What sample types and volumes are accepted for this QC assay?
We accept either extracted DNA (minimum 2 µL at 5 ng/µL) or 5 mL of whole blood collected in an EDTA tube. The assay is compatible with DNA extracted from fresh or frozen blood, and samples should be transported at 2–8°C without freezing.
UAE Regulatory & Data Privacy Adherence
Regulatory Compliance
Your data is protected under Federal Decree-Law No. 45 of 2021 on Personal Data Protection (PDPL) and Federal Law No. 2 of 2019 Concerning the Use of Information and Communication Technology in Health Fields. All genomic and personal information is encrypted, access-controlled, and never shared without explicit consent. This laboratory strictly adheres to DHA licensing requirements and ISO 9001:2015 standards for quality management.
For any privacy concerns, contact our Data Protection Officer at dpo@dnalabsuae.com or call +971 4 123 4567.
Clinical & Logistical Metadata
| Test Name | Sequencing Library QC‑qPCR (NGS Library QC) |
| Price (AED) | 350 AED |
| Turnaround Time | 1 week (from sample receipt to report) |
| Sample Type / Matrix | Extracted DNA (≥2 µL at 5 ng/µL) or 5 mL whole blood in EDTA |
| Methodology Used | Real-Time qPCR (SYBR Green or Probe) |
| ICD-10-CM Code | Z01.89 (Encounter for other specified special examinations) |
| LOINC Code | 96972-4 (NGS library quality control by qPCR) |
| DHA Facility License & Laboratory Address Invariants | License No. 1143 | Premises 105, Floor 1, Building 33, Dubai Healthcare City, Dubai, UAE | DNA Labs UAE |
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