Test Price
450 AED✅ Home Collection Available
Immunophenotyping by Flow Cytometry CD49d Test in UAE | 450 AED | DHA Licensed
Executive Summary & Core Metrics
The CD49d flow cytometry immunophenotyping test provides high-precision risk stratification for chronic lymphocytic leukemia (CLL) by quantifying CD49d surface antigen expression on malignant B-cells. This assay delivers a diagnostic sensitivity exceeding 99% under ISO 9001:2015 certified protocols and is conducted exclusively within accredited hospital facilities to ensure specimen integrity and patient safety.
- Diagnostic Precision: High-resolution 4-color fluorescent flow cytometry with standardized gating and calibration controls.
- Clinical Actionability: CD49d positivity correlates directly with aggressive CLL course and shorter treatment-free survival, guiding therapeutic intensity.
- Medical Oversight: Post-result tele-consultation with a specialist diagnostic radiologist for comprehensive result interpretation.
- Insurance & Support: Verify direct insurance coverage via WhatsApp at +971 54 548 8731.
Test Overview & Methodology
Immunophenotyping by flow cytometry for CD49d measures the expression density of the CD49d integrin chain on the surface of circulating lymphocytes. Elevated CD49d expression is an established independent prognostic marker in CLL, associated with shorter time to first treatment and inferior overall survival. The assay employs a phycoerythrin-conjugated CD49d monoclonal antibody clone L25 and multi-parameter scatter gating to resolve the neoplastic B-cell population from background lymphocytes. Results are reported as percentage positivity and mean fluorescence intensity ratio, interpreted alongside clinical staging and IGHV mutation status.
| Feature | CD49d Flow Cytometry | Standard Immunophenotyping |
|---|---|---|
| Precision | CD49d monoclonal antibody clone L25; high-resolution scatter gating with 4-color fluorescence | Generic lymphocyte panel without CD49d; reduced prognostic specificity |
| Methodology | 4-color fluorescent flow cytometry with standardized calibration and internal controls | 2-color or 3-color flow cytometry with variable calibration protocols |
| Turnaround | Same-day report when sample arrives at laboratory by 9 AM | 24-72 hours; frequently outsourced to external laboratories |
| Prognostic Value | CD49d positivity directly linked to aggressive CLL course and shorter treatment-free survival | Indirect assessment; may fail to capture CD49d-specific risk stratification |
Physician Insight & Safety Protocols
Mr. Prabhakar Reddy Kalathoor, Specialist Diagnostic Radiology (DHA Registration ID: 61713011), comments: "CD49d expression level is a well-validated independent prognosticator in CLL. A high CD49d-positive fraction suggests a more aggressive disease trajectory and warrants closer monitoring and earlier therapeutic intervention. This marker must always be interpreted alongside the complete clinical picture including Rai or Binet staging, IGHV mutational status, and cytogenetic abnormalities. No treatment decision should rely on CD49d expression alone – comprehensive clinical integration is mandatory."
Clinical Advisory: Medication Continuity
Do not discontinue prescribed therapy without consulting your treating physician
Abrupt cessation of anticoagulants, immunomodulators, or antineoplastic agents prior to sample collection may lead to thromboembolic events, disease flare, or acute withdrawal syndromes. Always coordinate medication management with the referring clinician before the procedure.
Exclusion Criteria & Emergency Indicators
- Active systemic infection with core temperature above 38°C
- Hemoglobin level below 7 g/dL requiring transfusion support
- Active hemorrhage or therapeutic anticoagulation with international normalized ratio above 3.0
- Known hypersensitivity to sodium heparin or latex-containing medical devices
- Minors under 18 years of age without parental consent per UAE Pediatric Healthcare Regulations
- Seek emergency care if: sudden severe pain at extraction site, expanding hematoma, or signs of systemic sepsis develop within 48 hours post-procedure
Patient FAQ & Clinical Guidance
1. What clinical information does the CD49d test provide about my CLL prognosis?
The CD49d test measures the percentage of CLL cells that express the CD49d surface protein. A high expression level (typically 30% or greater) signals a more aggressive leukemia subtype, enabling your oncologist to customize treatment intensity and surveillance frequency. This test works in concert with IGHV mutation analysis and FISH cytogenetics to build a complete prognostic picture.
2. How is the bone marrow sample collected and where does the procedure take place?
Hospital Extraction Only – Sample collection is conducted strictly within an accredited hospital facility; mobile home phlebotomy is disabled for safety. A qualified hematologist or oncologist performs a bone marrow aspiration and biopsy under sterile conditions, typically from the posterior iliac crest, using local anesthesia. Peripheral blood is drawn simultaneously via standard venipuncture. The entire procedure takes approximately 20 to 30 minutes.
3. When will I receive my CD49d test results and how are they communicated?
Same-day reporting is guaranteed if the hospital sends the sample to our laboratory by 9 AM. The flow cytometry report is delivered electronically to your referring physician, and a tele-consultation with a specialist diagnostic radiologist is available to walk you through the findings and answer any questions about next steps.
4. Are there any dietary or medication preparations required before the procedure?
No fasting is required for the peripheral blood component. However, inform your hematologist about all prescription medications, especially anticoagulants (warfarin, apixaban, rivaroxaban) and antiplatelet agents, as these may need to be temporarily paused under medical supervision to reduce bleeding risk during bone marrow aspiration.
UAE Regulatory & Data Privacy Adherence
This diagnostic service operates in full compliance with UAE federal healthcare data protection and medical liability frameworks. All patient health information is processed under the provisions of Federal Decree-Law No. 45 of 2021 on Personal Data Protection (PDPL) and Federal Law No. 2 of 2019 Concerning the Use of Information and Communication Technology in Health Fields. Clinical safety and informed consent protocols adhere to Federal Decree-Law No. 4 of 2016 on Medical Liability. The laboratory facility holds ISO 9001:2015 certification (Cert: INT/EGQ/2509DA/3139) and is subject to annual inspection by the Dubai Health Authority.
Pre-logistics guidance for hospital staff: Collect 3 mL whole blood in a sodium heparin tube and 2 mL bone marrow aspirate in a separate sodium heparin tube. Transport promptly at 18-22°C ambient temperature. Do not refrigerate or freeze. Label each tube with patient full name, medical record number, collection date and time, and clinical indication. Complete the requisition form with relevant history and current medication list.
WhatsApp support and insurance verification: +971 54 548 8731
Clinical & Logistical Metadata
| Test Name | Immunophenotyping by Flow Cytometry CD49d |
| Price (AED) | 450 AED |
| Turnaround Time | Same-day report when sample arrives by 9 AM |
| Sample Type / Matrix | Peripheral whole blood (sodium heparin) and bone marrow aspirate (sodium heparin). Hospital extraction only – collection conducted within accredited hospital facility; mobile home phlebotomy is disabled for safety. |
| Methodology Used | 4-color fluorescent flow cytometry using CD49d monoclonal antibody clone L25; forward and side scatter gating with isotype control; percentage positivity and mean fluorescence intensity ratio reported. |
| ICD-10-CM Code | C91.1 (Chronic lymphocytic leukemia of B-cell type) |
| LOINC Code | 48796-9 (Cells.CD49d in Blood by Flow cytometry) |
| DHA Facility License & Laboratory Address | License No. 1143 | Premises 105, Floor 1, Building 33, Dubai Healthcare City, Dubai, UAE | DNA Labs UAE |
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