Test Price
2,500 AED✅ Home Collection Available
Illumina-Based Bacterial Genome De Novo Assembly and Annotation
Executive Summary & Core Metrics
Bacterial genome de novo assembly and annotation via Illumina sequencing provides a comprehensive, high-resolution blueprint of a bacterial pathogen’s entire genetic makeup, enabling precise strain identification, virulence factor detection, and antibiotic resistance profiling for acute clinical decision-making. In the UAE, this advanced molecular test bridges epidemiological surveillance and personalized infection management. Key metrics: Price 2500 AED, Turnaround Time 4 weeks, Sample Type: Bacterial isolate (pure culture from clinical specimen).
Test Overview & Methodology
The Illumina-based de novo assembly workflow begins with high-quality genomic DNA extracted from a pure bacterial culture. The DNA is fragmented, adapter-ligated, and sequenced on an Illumina platform to generate >100x depth coverage. Raw reads are assembled using SPAdes, and the resulting contigs are annotated with Prokka to identify coding sequences, resistance genes, virulence factors, and mobile genetic elements. The final report includes strain-level typing (e.g., MLST), antimicrobial resistance (AMR) gene profiles, and plasmid content.
| Attribute | Our Test (Illumina De Novo Assembly) | Closest Alternative (Sanger 16S rRNA) |
|---|---|---|
| Precision / Coverage | Whole-genome, >100x depth, SNPs/indels, plasmids | Single gene (1.5 kb), genus-level only |
| Method & Resolution | SPAdes assembly + Prokka annotation, strain-level | PCR + capillary electrophoresis, no resistance gene |
| Turnaround Time | 4 weeks (complete report) | 3–5 days (limited data) |
Physician Insight & Safety Protocols
“As a Consultant Medical Genetics, I often guide clinicians and patients through the 4-week waiting period for comprehensive genomic profiling. The depth of information—resistance genes, virulence factors, and strain relatedness—far exceeds standard culture. However, results must always be correlated with clinical presentation and local epidemiology. This test is a powerful adjunct, not a standalone decision tool.”
— Lina Osama Zaki Quteineh, Consultant Medical Genetics, DHA Registration ID 9294403
Advisory: Medication & Treatment Continuity
Medication Warning: Do not discontinue prescribed antibiotics or any medication without consulting your treating physician. Genomic results may inform future changes but should not prompt immediate self-adjustment.
Exclusion Criteria & Emergency Red Flags
Exclusion Criteria & Emergency Red Flags
- Active severe coagulopathy or recent major surgery – please inform our team before sample collection.
- Current acute febrile illness with suspected sepsis (defined by qSOFA ≥2) – proceed to nearest emergency department immediately, do not delay for scheduling.
- If you experience high fever ≥39°C, confusion, rapid breathing, or severe localized pain, seek urgent medical care; these may indicate life-threatening infection requiring immediate empirical treatment.
Patient FAQ & Clinical Guidance
1. What is the primary clinical benefit of bacterial whole genome sequencing over traditional culture methods?
Illumina-based de novo assembly reveals antibiotic resistance genes, virulence factors, and strain-level relationships in a single assay, enabling targeted therapy and outbreak containment. This comprehensive genomic picture supports precision medicine and infection control in the UAE healthcare setting.
2. How should I prepare for the sample collection?
If a blood culture is ordered, no special preparation is needed—maintain normal diet and hydration. Inform the phlebotomist of any anticoagulant medications. For other clinical specimens (sputum, urine, swab), follow your physician’s specific instructions. Our VIP mobile phlebotomy service (available 8 AM to 11 PM) can collect blood cultures at your home or office.
3. Can the results be used to guide immediate antibiotic changes without consulting a doctor?
No, genomic findings must be interpreted by an infectious disease specialist or clinical microbiologist. Never adjust antibiotics without professional guidance to avoid resistance development and treatment failure. The final report includes expert commentary to aid your physician.
UAE Regulatory & Data Privacy Adherence
DNA Labs UAE operates under strict compliance with UAE federal data protection laws. All genomic data generated from Illumina sequencing is handled in accordance with Federal Decree-Law No. 45 of 2021 on Personal Data Protection (PDPL) and Federal Law No. 2 of 2019 Concerning the Use of Information and Communication Technology in Health Fields. Clinical safety and informed consent procedures align with Federal Decree-Law No. 4 of 2016 on Medical Liability. Your samples and genomic information are encrypted, access-controlled, and used solely for diagnostic purposes as authorised by you and your physician.
Clinical & Logistical Metadata
| Test Name | Illumina-Based Bacterial Genome De Novo Assembly and Annotation |
| Price (AED) | 2,500 AED |
| Turnaround Time | 4 weeks (complete report including annotation) |
| Sample Type / Matrix | Bacterial isolate (pure culture from blood, sputum, urine, or other clinical specimen); VIP Mobile Phlebotomy & Temperature-Controlled Cold-Chain Home Collection available daily 8 AM–11 PM for blood culture specimen. |
| Methodology Used | Illumina NGS (≥100x coverage), SPAdes de novo assembly, Prokka annotation, MLST typing, AMR gene and virulence factor detection |
| ICD-10-CM Code | B96.89 (Other specified bacterial agents as the cause of diseases classified elsewhere) |
| LOINC Code | 94061-3 (Bacterial whole genome sequencing) |
| DHA Facility License & Laboratory Address | DHA Facility License Number: 1143 | Premises 105, Floor 1, Building 33, Dubai Healthcare City, Dubai, UAE | Corporate Lab Branding: DNA Labs UAE |
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