Bacterial Stranded Transcriptome Library Preparation in UAE | 2100 AED | DHA Licensed

Executive Summary & Core Metrics

Precision Transcriptomics Service – Key Metrics

Accuracy Guarantee: 99.9% diagnostic sensitivity via ISO 9001:2015 accredited processing (Cert: INT/EGQ/2509DA/3139).
Premium Logistics: Hospital-grade home collection with ISO-certified cold-chain transport and VIP mobile phlebotomy, available daily from 8 AM to 11 PM.
Clinical Guidance: Post-test telephonic result interpretation by DHA-licensed genetic specialists.
Insurance: Direct billing verification via WhatsApp +971 54 548 8731.
Price: 2,100 AED | Turnaround Time: 7 days from sample receipt.

Test Overview & Methodology

Bacterial stranded transcriptome library preparation converts extracted bacterial RNA into high-fidelity, strand-specific sequencing libraries, enabling precise quantification of gene expression and antisense regulation. This advanced step ensures your research or clinical investigation captures the full transcriptomic landscape with directionality, essential for antimicrobial resistance profiling and functional genomics in the UAE’s healthcare and bioscience sectors.

Our protocol integrates rRNA depletion (Ribo‑Zero Plus), unique molecular identifiers (UMIs), and duplex error correction, processed on the Illumina NovaSeq X Plus platform – delivering results within 1 week from sample receipt.

Feature	Our Test (Precision Service)	Closest Alternative
Methodology	Stranded total RNA‑seq with rRNA depletion, UMIs & Illumina NovaSeq X Plus	Unstranded RNA‑seq without UMI or rRNA depletion
Turnaround Time	7 Days	14–21 Days
Strand Specificity	Yes – preserves transcript orientation	No – loses directionality, limits operon/antisense analysis
Error Correction	Duplex consensus calling (error rate < 0.001%)	Basic base‑calling only

Physician Insight & Safety Protocols

“Transcriptomic data provide a powerful window into bacterial gene regulation, but results must always be correlated with clinical findings, culture data, and antimicrobial susceptibility patterns. This library preparation is a research-grade tool; confirmatory diagnostic testing should follow clinical guidelines.”

— Lina Osama Zaki Quteineh, Consultant Medical Genetics, DHA License: 9294403

Advisory Precautions

Patients should continue all prescribed medications unless explicitly instructed otherwise by their treating physician. Transcriptome analysis provides complementary molecular data and is not intended to replace standard microbiological diagnostics or clinical assessment.

Exclusion Criteria & Emergency Red Flags

Exclusion Criteria: Patients with coagulopathies (INR > 2.0 or platelets < 50,000/µL) may be unsuitable for whole blood collection. Insufficient starting DNA (< 50 ng) from extracted DNA will require recollection.
ER Red Flags – Seek immediate care if you experience: confusion, chest pain, difficulty breathing, bluish lips, or severe fever with hypotension alongside any signs of bacterial infection.

Patient FAQ & Clinical Guidance

1. What is bacterial stranded transcriptome library preparation?

It is a process that converts bacterial RNA into directional sequencing libraries, capturing gene expression with strand specificity. This allows precise measurement of both coding and antisense transcripts, critical for operon analysis and antimicrobial resistance studies.

2. How should I prepare for sample collection?

No fasting or special preparation is required for extracted DNA or whole blood collection; simply stay hydrated. Follow any specific instructions provided by your healthcare provider regarding specimen type (e.g., bacterial isolate, biopsy, or blood).

3. Why choose strand‑specific library preparation over standard RNA‑seq?

Stranded libraries preserve transcript orientation, enabling accurate quantification of antisense transcripts, identification of overlapping genes, and operon mapping. Standard unstranded RNA‑seq loses directionality and cannot distinguish between sense and antisense reads.

4. What is the turnaround time for results?

Results are delivered within 7 days of sample receipt. This includes library preparation, sequencing on NovaSeq X Plus, and bioinformatic analysis with a comprehensive report.

5. Can this test be performed on archival tissue samples?

Yes, but only if RNA integrity is preserved. Fresh or properly preserved bacterial isolates, PAXgene-stabilized blood, or RNAlater-stored specimens are preferred. FFPE tissue typically yields degraded RNA unsuitable for stranded library construction.

UAE Regulatory & Data Privacy Adherence

Data Privacy: Fully compliant with Federal Decree-Law No. 45 of 2021 on Personal Data Protection (PDPL).
Health ICT Compliance: Adheres to Federal Law No. 2 of 2019 Concerning the Use of Information and Communication Technology in Health Fields.
Medical Liability: Clinical safety and consent processes follow Federal Decree-Law No. 4 of 2016 on Medical Liability.
ISO Accreditation: Processing under ISO 9001:2015 Quality Management (Certificate: INT/EGQ/2509DA/3139).
Facility License: DHA License No. 1143 – Premises 105, Floor 1, Building 33, Dubai Healthcare City, Dubai, UAE.
Logistics: Home collection available 8 AM – 11 PM. All samples transported under ISO‑certified cold chain (2–8°C). Support: +971 54 548 8731 (WhatsApp).

Clinical & Logistical Metadata

Test Name	Bacterial Stranded Transcriptome Library Preparation
Price (AED)	2,100 AED
Turnaround Time	7 days from sample receipt
Sample Type / Matrix	Bacterial isolate, tissue biopsy, or whole blood (PAXgene Blood RNA Tube)
Methodology Used	Stranded total RNA-seq with rRNA depletion, UMIs, duplex error correction, Illumina NovaSeq X Plus
ICD-10-CM Code	Z14.8, Z13.79, Z01.89
LOINC Code	93334-1
DHA Facility License & Laboratory Address Invariants	Facility No. 1143 – Premises 105, Floor 1, Building 33, Dubai Healthcare City, Dubai, UAE. Corporate Lab: DNA Labs UAE