PacBio Sequel II Library Preparation in UAE | 12,500 AED | DHA Licensed Long-Read Sequencing Readiness

Executive Summary & Core Metrics

Accuracy Guarantee: 99.9% Diagnostic Sensitivity via ISO Accredited Processing (ISO 9001:2015 Cert: INT/EGQ/2509DA/3139).
Premium Logistics: VIP Mobile Phlebotomy & Temperature-Controlled Cold-Chain Home Collection available daily from 8 AM to 11 PM.
Clinical Guidance: Telephonic Post-Test Clinical Guidance with a DHA-licensed Consultant Medical Genetics to interpret library quality and downstream implications.
Insurance: Direct Billing Verification via WhatsApp at +971 54 548 8731.

Test Overview & Methodology

The PacBio Sequel II Library Preparation service generates high-fidelity SMRTbell libraries for long-read sequencing, enabling comprehensive genomic analysis. Utilizing advanced size-selection and pooling protocols, this assay achieves library quality scores exceeding Q30 (99.9% base accuracy) in routine production, supporting both research and clinical investigations. The process converts extracted DNA into SMRTbell templates optimized for the Sequel II instrument, producing reads averaging 15–20 kb with HiFi base quality for detection of structural variants, epigenetic modifications, and complex genomic rearrangements.

Feature	PacBio Sequel II Library Prep	Illumina Short-Read Library Prep
Methodology	SMRTbell Template Preparation with Size Selection, Pooling, and Long-Read Sequencing Readiness	Standard end-repair, A-tailing, and ligation for short-read (<300 bp) platforms
Turnaround Time	20 Business Days	10–14 Business Days
Accuracy / Read Length	99.9% Consensus Accuracy, High-Molecular-Weight DNA Fragments up to 100 kb+	>99.9% Accuracy per base, limited to short fragments (up to 600 bp)

Physician Insight & Safety Protocols

“As a Consultant Medical Genetics, I advise that this library preparation serves as a critical starting point for genomic investigations and must be interpreted in conjunction with your full clinical history and phenotypic data. Any genetic findings should be discussed with a qualified genetic counselor to accurately assess clinical significance.” – Lina Osama Zaki Quteineh, Consultant Medical Genetics, DHA Registration ID: 9294403.

Advisory Precautions

Do not discontinue prescribed medication without consulting your doctor.

Exclusion Criteria & Emergency Red Flags

Inability to provide high-quality extracted DNA (e.g., severely degraded specimens) or active skin infection at the phlebotomy site (for whole blood collection).
Known allergy to latex or venipuncture supplies (alternative protocol may be arranged).
Seek immediate medical attention if you experience: fainting, persistent bleeding, severe pain, swelling, or signs of infection after blood draw.

Patient FAQ & Clinical Guidance

1. What is PacBio Sequel II Library Preparation and why is it used?

PacBio Sequel II Library Preparation converts extracted DNA into SMRTbell templates for high-accuracy long-read sequencing, enabling detection of structural variants, epigenetic modifications, and complex genomic rearrangements. The process includes DNA shearing, end-repair, adapter ligation, and size selection to generate libraries optimized for the Sequel II instrument, resulting in reads averaging 15–20 kb with HiFi base quality.

2. How should I prepare for my home blood collection appointment?

No fasting is required; simply hydrate normally and have your Emirates ID ready for verification. A trained phlebotomist will collect 5 mL of blood in a K2EDTA tube under cold-chain conditions, and you will receive a collection confirmation with sample ID.

3. What do I need to know about the 20-day turnaround time and my results?

The 20-day turnaround includes library construction, quality control, and sequencing readiness; final data is delivered through our secure online portal with optional telehealth interpretation. Your results will include library yield, fragment size distribution, and a sequencing-ready certification; downstream sequencing and bioinformatics analysis are booked separately.

4. Is this test covered by insurance in the UAE?

Coverage depends on your individual insurance plan. We offer direct billing verification through WhatsApp at +971 54 548 8731. Please contact us with your policy details to confirm eligibility and any prior authorization requirements before scheduling your collection.

UAE Regulatory & Data Privacy Adherence

This diagnostic service complies fully with Federal Decree-Law No. 45 of 2021 on Personal Data Protection (PDPL) governing the processing of personal data and Federal Law No. 2 of 2019 Concerning the Use of Information and Communication Technology in Health Fields ensuring secure digital health information exchange. All clinical procedures adhere to Federal Decree-Law No. 4 of 2016 on Medical Liability to guarantee patient safety and informed consent. DNA Labs UAE is a DHA-licensed facility (License No. 1143) operating under the regulatory oversight of the Dubai Health Authority.

Clinical & Logistical Metadata

Test Name	PacBio Sequel II Library Preparation
Price (AED)	12,500 AED
Turnaround Time	20 Business Days
Sample Type / Matrix	Whole Blood (5 mL in K2EDTA tube) – VIP Mobile Phlebotomy & Temperature-Controlled Cold-Chain Home Collection available daily from 8 AM to 11 PM
Methodology Used	SMRTbell Template Preparation with Size Selection, Pooling, and Long-Read Sequencing Readiness
ICD-10-CM Code	Z13.89
LOINC Code	51960-2
DHA Facility License & Laboratory Address	DHA Facility License Number: 1143 \| Premises 105, Floor 1, Building 33, Dubai Healthcare City, Dubai, UAE – DNA Labs UAE

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