EPIDERMAL PEMPHIGUS ANTIBODY IFA Test
Test Name: EPIDERMAL PEMPHIGUS ANTIBODY IFA Test
Components: Price 470.0 AED
Sample Condition: 2 mL (1 mL min.) serum from 1 SST. Ship refrigerated or frozen. Overnight fasting is preferred.
Report Delivery: Sample Daily by 11 am; Report Same day
Method: Immunofluorescence Assay
Test Type: Disorders of Skin
Doctor: Dermatologist
Test Department: IMMUNOPATHOLOGY
Pre Test Information: Overnight fasting is preferred.
Test Details
The EPIDERMAL (PEMPHIGUS) ANTIBODY IFA test is a diagnostic test used to detect antibodies in the blood that are associated with pemphigus, a group of autoimmune blistering disorders that affect the skin and mucous membranes.
The test involves collecting a blood sample from the patient and then performing an indirect immunofluorescence assay (IFA) on the sample. In this assay, the patient’s serum is exposed to a substrate that contains monkey esophagus or human skin tissue. If the patient has antibodies against the epidermal antigens, they will bind to the substrate.
After washing away any unbound antibodies, a fluorescently labeled secondary antibody is added. This secondary antibody will bind to any antibodies that have bound to the substrate.
The sample is then examined under a fluorescence microscope. If the patient has pemphigus, a characteristic pattern of fluorescence will be observed, indicating the presence of epidermal antibodies.
The EPIDERMAL (PEMPHIGUS) ANTIBODY IFA test is a valuable tool in diagnosing pemphigus and differentiating it from other blistering disorders. It can also be used to monitor disease activity and response to treatment.
| Test Name | EPIDERMAL PEMPHIGUS ANTIBODY IFA Test |
|---|---|
| Components | |
| Price | 470.0 AED |
| Sample Condition | 2 mL (1 mL min.) serum from 1 SST. Ship refrigerated or frozen. Overnight fasting is preferred. |
| Report Delivery | Sample Daily by 11 am; Report Same day |
| Method | Immunofluorescence Assay |
| Test type | Disorders of Skin |
| Doctor | Dermatologist |
| Test Department: | IMMUNOPATHOLOGY |
| Pre Test Information | Overnight fasting is preferred. |
| Test Details | The EPIDERMAL (PEMPHIGUS) ANTIBODY IFA test is a diagnostic test used to detect antibodies in the blood that are associated with pemphigus, a group of autoimmune blistering disorders that affect the skin and mucous membranes. The test involves collecting a blood sample from the patient and then performing an indirect immunofluorescence assay (IFA) on the sample. In this assay, the patient’s serum is exposed to a substrate that contains monkey esophagus or human skin tissue. If the patient has antibodies against the epidermal antigens, they will bind to the substrate. After washing away any unbound antibodies, a fluorescently labeled secondary antibody is added. This secondary antibody will bind to any antibodies that have bound to the substrate. The sample is then examined under a fluorescence microscope. If the patient has pemphigus, a characteristic pattern of fluorescence will be observed, indicating the presence of epidermal antibodies. The EPIDERMAL (PEMPHIGUS) ANTIBODY IFA test is a valuable tool in diagnosing pemphigus and differentiating it from other blistering disorders. It can also be used to monitor disease activity and response to treatment. |
