SMOOTH MUSCLE ANTIBODY ASMA IFA Test
Cost: AED 400.0
Test Details
The Smooth Muscle Antibody (ASMA) IFA test is a laboratory test used to detect the presence of smooth muscle antibodies in the blood. These antibodies are associated with certain autoimmune diseases, particularly autoimmune hepatitis and some cases of chronic active hepatitis.
Components
Price: 400.0 AED
Sample Condition
2 mL (1 mL min.) serum from 1 SST. Ship refrigerated or frozen. Overnight fasting is preferred.
Report Delivery
Sample: Daily by 9 am
Report: Same day
Method
Immunofluorescence Assay
Test Type
Autoimmune disorders – Chronic liver disease
Doctor
Gastroenterologist
Test Department
Immunopathology
Pre Test Information
Overnight fasting is preferred.
Test Procedure
The Smooth Muscle Antibody (ASMA) IFA test is performed using an indirect immunofluorescence assay (IFA) technique. A blood sample is collected from the patient and the serum is separated. The serum is then mixed with a substrate that contains smooth muscle tissue. If the patient has smooth muscle antibodies in their blood, these antibodies will bind to the smooth muscle tissue in the substrate. After incubation, the substrate is washed to remove any unbound antibodies. A fluorescently labeled secondary antibody is then added, which binds to any smooth muscle antibodies that are bound to the substrate. The substrate is washed again to remove any unbound secondary antibodies. Finally, the substrate is examined under a fluorescent microscope. If smooth muscle antibodies are present in the patient’s blood, the smooth muscle tissue in the substrate will fluoresce, indicating a positive result.
Diagnostic Significance
The Smooth Muscle Antibody (ASMA) IFA test is a useful tool in diagnosing autoimmune hepatitis and differentiating it from other liver diseases. It is important to note that a positive result does not necessarily confirm the presence of autoimmune hepatitis, as these antibodies can also be found in other conditions. Therefore, the test is usually used in conjunction with other clinical and laboratory findings to make a definitive diagnosis.
| Test Name | SMOOTH MUSCLE ANTIBODY ASMA IFA Test |
|---|---|
| Components | |
| Price | 400.0 AED |
| Sample Condition | 2 mL (1 mL min.) serum from 1 SST. Shiprefrigeratedorfrozen.Overnight fastingispreferred. |
| Report Delivery | Sample Daily by 9 am;Report Same day |
| Method | Immunofluorescence Assay |
| Test type | Autoimmune disorders-Chronic liver disease |
| Doctor | Gastroenterologist |
| Test Department: | IMMUNOPATHOLOGY |
| Pre Test Information | Overnight fasting is preferred. |
| Test Details |
The Smooth Muscle Antibody (ASMA) IFA test is a laboratory test used to detect the presence of smooth muscle antibodies in the blood. These antibodies are associated with certain autoimmune diseases, particularly autoimmune hepatitis and some cases of chronic active hepatitis. The test is performed using an indirect immunofluorescence assay (IFA) technique. A blood sample is collected from the patient and the serum is separated. The serum is then mixed with a substrate that contains smooth muscle tissue. If the patient has smooth muscle antibodies in their blood, these antibodies will bind to the smooth muscle tissue in the substrate. After incubation, the substrate is washed to remove any unbound antibodies. A fluorescently labeled secondary antibody is then added, which binds to any smooth muscle antibodies that are bound to the substrate. The substrate is washed again to remove any unbound secondary antibodies. Finally, the substrate is examined under a fluorescent microscope. If smooth muscle antibodies are present in the patient’s blood, the smooth muscle tissue in the substrate will fluoresce, indicating a positive result. The Smooth Muscle Antibody (ASMA) IFA test is a useful tool in diagnosing autoimmune hepatitis and differentiating it from other liver diseases. It is important to note that a positive result does not necessarily confirm the presence of autoimmune hepatitis, as these antibodies can also be found in other conditions. Therefore, the test is usually used in conjunction with other clinical and laboratory findings to make a definitive diagnosis. |
