IMMUNOHISTOCHEMISTRY CD68 Test
At DNA Labs UAE, we offer the IMMUNOHISTOCHEMISTRY CD68 Test for cancer diagnosis. This test helps detect specific proteins in tissue sections using antibodies. CD68 is a commonly used marker for macrophages and monocytes.
Test Details
The CD68 immunohistochemistry test involves the following steps:
- Tissue Preparation: The tissue sample is collected and processed for histological examination. It is embedded in paraffin or frozen for sectioning.
- Sectioning: Thin sections (usually around 4-5 micrometers thick) of the tissue are cut using a microtome and mounted onto glass slides.
- Deparaffinization (if using paraffin-embedded tissue): If the tissue is paraffin-embedded, the slides are deparaffinized by immersing them in xylene or other clearing agents, followed by rehydration through a graded series of alcohol.
- Antigen Retrieval: In order to expose the CD68 antigen for antibody binding, antigen retrieval is performed. This can be done by heat-induced epitope retrieval (HIER) using heat or enzymatic digestion.
- Blocking: Non-specific binding sites on the tissue section are blocked using a blocking solution, usually containing normal serum or protein blocking agents.
- Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific to CD68. The primary antibody binds to the CD68 antigen present on macrophages and monocytes in the tissue.
- Washing: After incubation with the primary antibody, the slides are washed to remove any unbound antibody.
- Secondary Antibody Incubation: A secondary antibody, conjugated to a detection system (such as an enzyme or a fluorochrome), is applied to the tissue sections. The secondary antibody binds to the primary antibody, allowing for the visualization of the CD68-positive cells.
- Washing: The slides are washed again to remove any unbound secondary antibody.
- Visualization: The presence of CD68-positive cells is visualized using a detection system appropriate for the secondary antibody used. This can be achieved through enzymatic reactions or fluorescence microscopy.
- Counterstaining: In some cases, a counterstain, such as hematoxylin, is applied to provide contrast and enhance the visualization of the tissue structure.
- Mounting: The slides are coverslipped using an appropriate mounting medium.
Test Name: IMMUNOHISTOCHEMISTRY CD68 Test
Components
Price: 410.0 AED
Sample Condition
Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy, and Clinical history.
Report Delivery
Sample Daily by 6 pm; Report Block: 5 days, Tissue Biopsy: 5 days, Tissue large complex: 7 days
Method
Immunohistochemistry
Test Type
Cancer
Doctor
Oncologist, Pathologist
Test Department
Pre Test Information: Provide a copy of the Histopathology report, Site of biopsy, and Clinical history.
Interpreting the Results
The results of the CD68 immunohistochemistry test can be interpreted by examining the stained tissue sections under a microscope. CD68-positive cells will appear brown (in the case of enzyme-based detection) or fluorescent (in the case of fluorescence-based detection) in the tissue. The intensity and distribution of CD68 staining can provide information about the presence and localization of macrophages and monocytes in the tissue sample.
| Test Name | IMMUNOHISTOCHEMISTRY CD68 Test |
|---|---|
| Components | |
| Price | 410.0 AED |
| Sample Condition | Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history. |
| Report Delivery | Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days |
| Method | Immunohistochemistry |
| Test type | Cancer |
| Doctor | Oncologist, Pathologist |
| Test Department: | |
| Pre Test Information | Provide a copy of the Histopathology report, Site of biopsy and Clinical history. |
| Test Details | Immunohistochemistry (IHC) is a technique used to detect specific proteins in tissue sections using antibodies. CD68 is a commonly used marker for macrophages and monocytes. The CD68 test in immunohistochemistry involves the use of an antibody against CD68 to identify and visualize macrophages and monocytes in tissue samples. To perform the CD68 immunohistochemistry test, the following steps are typically followed: 1. Tissue Preparation: The tissue sample is collected and processed for histological examination. It is embedded in paraffin or frozen for sectioning. 2. Sectioning: Thin sections (usually around 4-5 micrometers thick) of the tissue are cut using a microtome and mounted onto glass slides. 3. Deparaffinization (if using paraffin-embedded tissue): If the tissue is paraffin-embedded, the slides are deparaffinized by immersing them in xylene or other clearing agents, followed by rehydration through a graded series of alcohol. 4. Antigen Retrieval: In order to expose the CD68 antigen for antibody binding, antigen retrieval is performed. This can be done by heat-induced epitope retrieval (HIER) using heat or enzymatic digestion. 5. Blocking: Non-specific binding sites on the tissue section are blocked using a blocking solution, usually containing normal serum or protein blocking agents. 6. Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific to CD68. The primary antibody binds to the CD68 antigen present on macrophages and monocytes in the tissue. 7. Washing: After incubation with the primary antibody, the slides are washed to remove any unbound antibody. 8. Secondary Antibody Incubation: A secondary antibody, conjugated to a detection system (such as an enzyme or a fluorochrome), is applied to the tissue sections. The secondary antibody binds to the primary antibody, allowing for the visualization of the CD68-positive cells. 9. Washing: The slides are washed again to remove any unbound secondary antibody. 10. Visualization: The presence of CD68-positive cells is visualized using a detection system appropriate for the secondary antibody used. This can be achieved through enzymatic reactions or fluorescence microscopy. 11. Counterstaining: In some cases, a counterstain, such as hematoxylin, is applied to provide contrast and enhance the visualization of the tissue structure. 12. Mounting: The slides are coverslipped using an appropriate mounting medium. The results of the CD68 immunohistochemistry test can be interpreted by examining the stained tissue sections under a microscope. CD68-positive cells will appear brown (in the case of enzyme-based detection) or fluorescent (in the case of fluorescence-based detection) in the tissue. The intensity and distribution of CD68 staining can provide information about the presence and localization of macrophages and monocytes in the tissue sample. |
