HLA PRE-TRANSPLANT WORKUP NON SENSITIZED RECIPEINT PANEL1 Test
Welcome to DNA Labs UAE, where we offer comprehensive genetic testing services for transplant patients. In this blog post, we will discuss the HLA pre-transplant workup for non-sensitized recipients.
Test Components
The HLA PRE-TRANSPLANT WORKUP NON SENSITIZED RECIPEINT PANEL1 Test includes the following components:
- HLA DNA Typing for Kidney / Liver transplant, PCR Low resolution Luminex
- HLA T & B cell lymphocyte cross match vs potential donor
- HLA Panel Reactive IgG antibodies (PRA) Screen Luminex
Test Cost
The cost of the HLA PRE-TRANSPLANT WORKUP NON SENSITIZED RECIPEINT PANEL1 Test is AED 4490.0.
Sample Condition
The required sample condition for the recipient includes:
- 6 mL (3 mL min.) whole blood in 1 Yellow Top (ACD) tube
- 3 mL (2 mL min.) serum from 1 SST
- 4 mL (2 mL min.) whole blood in 1 Lavender top (EDTA) tube
Please collect the specimen preferably 48 hours after dialysis. Ship the samples refrigerated and do not freeze them. A doctor’s prescription on the letterhead with 1 photograph each of the recipient and donor, duly stamped, is required. Additionally, a Consent form for HLA Typing for Solid organ transplant (Form 33) is mandatory.
Report Delivery
The report for the HLA PRE-TRANSPLANT WORKUP NON SENSITIZED RECIPEINT PANEL1 Test will be delivered within 10-12 days.
Method
The test is performed using Complement Dependent Cytotoxicity with DTT treatment, Reverse SSOP – Luminex, and Luminex X-Map technology.
Test Type
The HLA PRE-TRANSPLANT WORKUP NON SENSITIZED RECIPEINT PANEL1 Test falls under the category of transplantation tests.
Doctor
The test is typically ordered by a Nephrologist.
Test Department
The HLA PRE-TRANSPLANT WORKUP NON SENSITIZED RECIPEINT PANEL1 Test is conducted in the Test Department.
Pre Test Information
Please collect the specimen preferably 48 hours after dialysis. A doctor’s prescription on the letterhead with 1 photograph each of the recipient and donor, duly stamped, is mandatory. Additionally, a Consent form for HLA Typing for Solid organ transplant (Form 33) is required.
Test Details
The HLA pre-transplant workup for a non-sensitized recipient typically includes the following tests:
- HLA typing: This test determines the recipient’s human leukocyte antigen (HLA) tissue type. HLA matching between the donor and recipient is crucial for successful transplantation.
- Antibody screening: This test checks for the presence of pre-formed antibodies in the recipient’s blood. These antibodies can be directed against potential donor antigens and may increase the risk of rejection.
- Crossmatching: This test assesses the compatibility between the recipient’s antibodies and the potential donor’s antigens. It helps identify any antibodies that may react against the donor’s HLA antigens.
- Viral screening: The recipient will be tested for various viral infections, including hepatitis B and C, HIV, cytomegalovirus (CMV), and Epstein-Barr virus (EBV). These tests ensure that the recipient does not have any active viral infections that could complicate the transplant procedure.
- Renal function tests: The recipient’s kidney function will be evaluated through blood tests, including serum creatinine, blood urea nitrogen (BUN), and estimated glomerular filtration rate (eGFR). These tests provide baseline information about the recipient’s kidney function.
- Imaging studies: Imaging studies such as ultrasound or CT scan may be performed to assess the recipient’s kidneys and other relevant structures.
- Cardiac evaluation: The recipient may undergo a thorough cardiac evaluation, including an electrocardiogram (ECG) and echocardiogram, to assess their cardiac health and determine their suitability for surgery.
- Pulmonary function tests: These tests evaluate the recipient’s lung function and help assess their suitability for surgery under general anesthesia.
- Blood typing and crossmatching: The recipient’s ABO blood type will be determined, and blood crossmatching will be performed to ensure compatibility with potential donor blood.
- Medical history and physical examination: A detailed medical history and physical examination will be conducted to assess the recipient’s overall health and identify any potential risk factors for transplantation.
Please note that the specific tests may vary depending on the transplant center’s protocols and the individual recipient’s medical condition.
| Test Name | HLA PRE-TRANSPLANT WORKUP NON SENSITIZED RECIPEINT PANEL1 Test |
|---|---|
| Components | Recipient: *HLA DNA Typing for Kidney / Liver transplant, PCR Low resolution Luminex *HLA T & B cell lymphocyte cross match vs potential donor*HLA Panel Reactive IgG antibodies (PRA) Screen Luminex |
| Price | 4490.0 AED |
| Sample Condition | RECIPIENT: 6 mL (3 mL min.) whole blood in 1 Yellow Top (ACD) tube AND 3 mL (2 mL min.) serum from 1 SST AND 4 mL ( 2 mL min.) whole blood in 1 Lavender top (EDTA) tube. Collect specimen preferably 48 hours after dialysis. Ship refrigerated. DO NOT FREEZE. Doctor’s prescription on the letter head with 1 photograph each of recipient and donor duly stamped ANDConsent form for HLA Typing for Solid organ transplant (Form 33) is mandatory. |
| Report Delivery | 10-12 days |
| Method | Complement Dependent Cytotoxicity with DTT treatment, Reverse SSOP – Luminex, Luminex X-Map technology |
| Test type | Transplantation |
| Doctor | Nephrologist |
| Test Department: | |
| Pre Test Information | Collect specimen preferably 48 hours after dialysis. Doctor’s prescription on the letter head with 1 photograph each of recipient and donor duly stamped AND Consent form for HLA Typing for Solid organ transplant (Form 33) is mandatory. |
| Test Details |
The HLA pre-transplant workup for a non-sensitized recipient typically includes the following tests: 1. HLA typing: This test determines the recipient’s human leukocyte antigen (HLA) tissue type. HLA matching between the donor and recipient is crucial for successful transplantation. 2. Antibody screening: This test checks for the presence of pre-formed antibodies in the recipient’s blood. These antibodies can be directed against potential donor antigens and may increase the risk of rejection. 3. Crossmatching: This test assesses the compatibility between the recipient’s antibodies and the potential donor’s antigens. It helps identify any antibodies that may react against the donor’s HLA antigens. 4. Viral screening: The recipient will be tested for various viral infections, including hepatitis B and C, HIV, cytomegalovirus (CMV), and Epstein-Barr virus (EBV). These tests ensure that the recipient does not have any active viral infections that could complicate the transplant procedure. 5. Renal function tests: The recipient’s kidney function will be evaluated through blood tests, including serum creatinine, blood urea nitrogen (BUN), and estimated glomerular filtration rate (eGFR). These tests provide baseline information about the recipient’s kidney function. 6. Imaging studies: Imaging studies such as ultrasound or CT scan may be performed to assess the recipient’s kidneys and other relevant structures. 7. Cardiac evaluation: The recipient may undergo a thorough cardiac evaluation, including an electrocardiogram (ECG) and echocardiogram, to assess their cardiac health and determine their suitability for surgery. 8. Pulmonary function tests: These tests evaluate the recipient’s lung function and help assess their suitability for surgery under general anesthesia. 9. Blood typing and crossmatching: The recipient’s ABO blood type will be determined, and blood crossmatching will be performed to ensure compatibility with potential donor blood. 10. Medical history and physical examination: A detailed medical history and physical examination will be conducted to assess the recipient’s overall health and identify any potential risk factors for transplantation. It is important to note that the specific tests may vary depending on the transplant center’s protocols and the individual recipient’s medical condition. |
