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Hb Electrophoresis by HPLC Test

400 د.إ

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The Hemoglobin (Hb) Electrophoresis by High-Performance Liquid Chromatography (HPLC) test is a sophisticated diagnostic procedure employed to analyze and differentiate the various types of hemoglobin present in the blood. This test is particularly crucial for the diagnosis and management of hemoglobinopathies, such as sickle cell anemia, thalassemia, and other disorders associated with abnormal hemoglobin production or structure.

By utilizing HPLC technology, the Hb Electrophoresis test offers a high level of accuracy, sensitivity, and specificity. This method separates the different hemoglobin molecules based on their size, charge, and interaction with the chromatography medium. It provides a detailed profile of the hemoglobin variants, enabling healthcare professionals to make precise diagnoses and tailor treatment plans accordingly.

At DNA Labs UAE, this test is available for a cost of 400 AED. DNA Labs UAE is equipped with state-of-the-art facilities and expert personnel to ensure reliable and timely test results. This test is crucial for individuals with a family history of hemoglobinopathies or those exhibiting symptoms related to these conditions, facilitating early intervention and management.

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Hb Electrophorosis by HPLC Test

Test Name: Hb Electrophorosis by HPLC Test

Components: EDTA Vacutainer (2ml)

Price: 400.0 AED

Sample Condition: Peripheral blood

Report Delivery: 1 day

Method: Electrophoresis

Test type: Genetics

Doctor: General Physician

Test Department:

Pre Test Information: Hb Electrophorosis by HPLC can be done with a Doctor’s prescription. Prescription is not applicable for surgery and pregnancy cases or people planning to travel abroad.

Test Details:

Hb electrophoresis is a laboratory technique used to separate and identify different types of hemoglobin in the blood. High-performance liquid chromatography (HPLC) is a common method used for Hb electrophoresis. In HPLC, a liquid mobile phase carries the sample through a column packed with a stationary phase. As the sample passes through the column, different components in the sample interact with the stationary phase to varying degrees, leading to their separation.

In the case of Hb electrophoresis, the different types of hemoglobin present in the sample will have different affinities for the stationary phase, resulting in their separation. To perform Hb electrophoresis by HPLC, a blood sample is first collected from the patient. The red blood cells are then lysed to release the hemoglobin. The hemoglobin sample is then injected into the HPLC system, where it is carried through the column by the mobile phase. As the sample passes through the column, the different types of hemoglobin are separated based on their interactions with the stationary phase.

The separated hemoglobin components are detected and quantified by a detector, such as a UV/Vis detector, which measures the absorbance of the eluting compounds. The detector generates a chromatogram, which is a graphical representation of the separation, showing the different peaks corresponding to the different types of hemoglobin. By analyzing the chromatogram, the different types of hemoglobin present in the sample can be identified and quantified.

This information is important for diagnosing and monitoring various hemoglobinopathies and thalassemias, which are genetic disorders affecting the structure or production of hemoglobin. Overall, Hb electrophoresis by HPLC is a powerful technique for separating and analyzing different types of hemoglobin in blood samples, allowing for the diagnosis and monitoring of various hemoglobin disorders.

Test Name Hb Electrophorosis by HPLC Test
Components EDTA Vacutainer (2ml)
Price 400.0 AED
Sample Condition Peripheral blood
Report Delivery 1 day
Method Electrophoresis
Test type Genetics
Doctor General Physician
Test Department:
Pre Test Information Hb Electrophorosis by HPLC can be done with a Doctors prescription. Prescription is not applicable for surgery and pregnancy cases or people planing to travel abroad.
Test Details

Hb electrophoresis is a laboratory technique used to separate and identify different types of hemoglobin in the blood. High-performance liquid chromatography (HPLC) is a common method used for Hb electrophoresis.

In HPLC, a liquid mobile phase carries the sample through a column packed with a stationary phase. As the sample passes through the column, different components in the sample interact with the stationary phase to varying degrees, leading to their separation. In the case of Hb electrophoresis, the different types of hemoglobin present in the sample will have different affinities for the stationary phase, resulting in their separation.

To perform Hb electrophoresis by HPLC, a blood sample is first collected from the patient. The red blood cells are then lysed to release the hemoglobin. The hemoglobin sample is then injected into the HPLC system, where it is carried through the column by the mobile phase. As the sample passes through the column, the different types of hemoglobin are separated based on their interactions with the stationary phase.

The separated hemoglobin components are detected and quantified by a detector, such as a UV/Vis detector, which measures the absorbance of the eluting compounds. The detector generates a chromatogram, which is a graphical representation of the separation, showing the different peaks corresponding to the different types of hemoglobin.

By analyzing the chromatogram, the different types of hemoglobin present in the sample can be identified and quantified. This information is important for diagnosing and monitoring various hemoglobinopathies and thalassemias, which are genetic disorders affecting the structure or production of hemoglobin.

Overall, Hb electrophoresis by HPLC is a powerful technique for separating and analyzing different types of hemoglobin in blood samples, allowing for the diagnosis and monitoring of various hemoglobin disorders.

SKU: TEST-6531 Category:

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