ANTI ds DNA ANTIBODY CRITHIDIA IFA Test
Test Details
The anti dsDNA antibody Crithidia IFA (Indirect Immunofluorescence Assay) test is a diagnostic test used to detect the presence of anti-double-stranded DNA (dsDNA) antibodies in the blood. These antibodies are specific to systemic lupus erythematosus (SLE), an autoimmune disease.
In this test, Crithidia luciliae, a type of protozoan, is used as a substrate to detect the antibodies. The protozoan cells are fixed on a slide and then incubated with the patient’s serum. If the patient has anti-dsDNA antibodies in their blood, these antibodies will bind to the dsDNA present in the Crithidia cells.
After incubation, the slide is washed to remove any unbound antibodies, and then a fluorescent-labeled anti-human antibody is added. This secondary antibody binds to the patient’s anti-dsDNA antibodies if they are present. The slide is then examined under a fluorescence microscope, and if fluorescence is observed in the Crithidia cells, it indicates the presence of anti-dsDNA antibodies.
The Crithidia IFA test is considered a sensitive and specific test for detecting anti-dsDNA antibodies and is often used as a confirmatory test for SLE. However, it should be used in conjunction with other clinical and laboratory findings to make a definitive diagnosis of SLE.
Test Name
ANTI ds DNA ANTIBODY CRITHIDIA IFA Test
Components
Double Stranded / Native
Price
400.0 AED
Sample Condition
2 mL (1 mL min.) serum from 1 SST. Ship refrigerated or frozen. Overnight fasting is preferred.
Report Delivery
Sample Daily by 11 am; Report Same day
Method
Immunofluorescence Assay
Test Type
Autoimmune disorders
Doctor
Rheumatologist, Dermatologist, Physician
Test Department
IMMUNOPATHOLOGY
Pre Test Information
Overnight fasting is preferred.
| Test Name | ANTI ds DNA ANTIBODY CRITHIDIA IFA Test |
|---|---|
| Components | Double Stranded / Native |
| Price | 400.0 AED |
| Sample Condition | 2 mL (1 mL min.) serum from 1 SST. Ship refrigerated or frozen. Overnight fasting is preferred. |
| Report Delivery | Sample Daily by 11 am; Report Same day |
| Method | Immunofluorescence Assay |
| Test type | Autoimmune disorders |
| Doctor | Rheumatologist, Dermatologist, Physician |
| Test Department: | IMMUNOPATHOLOGY |
| Pre Test Information | Overnight fasting is preferred. |
| Test Details | The anti dsDNA antibody Crithidia IFA (Indirect Immunofluorescence Assay) test is a diagnostic test used to detect the presence of anti-double-stranded DNA (dsDNA) antibodies in the blood. These antibodies are specific to systemic lupus erythematosus (SLE), an autoimmune disease. In this test, Crithidia luciliae, a type of protozoan, is used as a substrate to detect the antibodies. The protozoan cells are fixed on a slide and then incubated with the patient’s serum. If the patient has anti-dsDNA antibodies in their blood, these antibodies will bind to the dsDNA present in the Crithidia cells. After incubation, the slide is washed to remove any unbound antibodies, and then a fluorescent-labeled anti-human antibody is added. This secondary antibody binds to the patient’s anti-dsDNA antibodies if they are present. The slide is then examined under a fluorescence microscope, and if fluorescence is observed in the Crithidia cells, it indicates the presence of anti-dsDNA antibodies. The Crithidia IFA test is considered a sensitive and specific test for detecting anti-dsDNA antibodies and is often used as a confirmatory test for SLE. However, it should be used in conjunction with other clinical and laboratory findings to make a definitive diagnosis of SLE. |
