ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test
At DNA Labs UAE, we offer the ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test for the detection of antibodies against double-stranded DNA (ds DNA) in a person’s blood. This test is performed using a technique called indirect immunofluorescence assay (IFA) and utilizes Crithidia luciliae, a type of parasite, as a substrate.
Test Details
The ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test is performed using a patient’s blood sample. The sample is mixed with Crithidia luciliae, and if the patient has antibodies against ds DNA, these antibodies will bind to the Crithidia luciliae. A fluorescent dye is then added, which attaches to the bound antibodies, causing them to emit fluorescence under a microscope. The presence and pattern of fluorescence indicate the presence of anti-ds DNA antibodies.
This test is usually performed in dilutions, starting with a high concentration of Crithidia luciliae and then serially diluted to lower concentrations. This allows for the determination of the antibody titer, which is the highest dilution at which the antibodies can still be detected.
Test Cost and Sample Condition
The cost of the ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test is AED 770.0. The sample condition required is 3 mL (1.5 mL min.) serum from 1 SST. The sample should be shipped refrigerated or frozen, and overnight fasting is preferred.
Report Delivery
The sample for the ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test should be provided by 11 am, and the report will be delivered the next day.
Test Type and Doctor
The ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test is a type of autoimmune disorders test. It is typically requested by rheumatologists, dermatologists, and physicians.
Test Department
The ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test is conducted in the Immunopathology department at DNA Labs UAE.
Pre Test Information
Overnight fasting is preferred before taking the ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test.
Diagnosis and Monitoring of Systemic Lupus Erythematosus (SLE)
The ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test is primarily used in the diagnosis and monitoring of systemic lupus erythematosus (SLE), an autoimmune disease. Anti-ds DNA antibodies are highly specific for SLE and are found in approximately 60-70% of patients with the disease. Higher antibody titers are associated with more active disease and can help guide treatment decisions.
It is important to note that this test is not used as a standalone diagnostic tool for SLE, as the presence of anti-ds DNA antibodies can also be seen in other autoimmune diseases and even some infectious diseases. Therefore, it is usually combined with other clinical and laboratory findings to make a definitive diagnosis.
| Test Name | ANTI – ds DNA ANTIBODY CRITHIDIA IFA IN DILUTIONS Test |
|---|---|
| Components | |
| Price | 770.0 AED |
| Sample Condition | 3 mL (1.5 mL min.) serum from 1 SST. Ship refrigerated or frozen. Overnight fasting is preferred. |
| Report Delivery | Sample Daily by 11 am; Report Next day |
| Method | Immunofluorescence Assay |
| Test type | Autoimmune disorders |
| Doctor | Rheumatologist, Dermatologist, Physician |
| Test Department: | IMMUNOPATHOLOGY |
| Pre Test Information | Overnight fasting is preferred. |
| Test Details | The anti-ds DNA antibody Crithidia IFA in dilutions test is a laboratory test used to detect the presence of antibodies against double-stranded DNA (ds DNA) in a person’s blood. This test utilizes a technique called indirect immunofluorescence assay (IFA) and uses Crithidia luciliae, a type of parasite, as a substrate. In this test, the patient’s blood sample is mixed with Crithidia luciliae, and if the patient has antibodies against ds DNA, these antibodies will bind to the Crithidia luciliae. Then, a fluorescent dye is added, which will attach to the bound antibodies, causing them to emit fluorescence under a microscope. The presence and pattern of fluorescence indicate the presence of anti-ds DNA antibodies. The test is usually performed in dilutions, meaning that the blood sample is initially mixed with a high concentration of Crithidia luciliae, and then serially diluted to lower concentrations. This allows for the determination of the antibody titer, which is the highest dilution at which the antibodies can still be detected. The anti-ds DNA antibody Crithidia IFA in dilutions test is primarily used in the diagnosis and monitoring of systemic lupus erythematosus (SLE), an autoimmune disease. Anti-ds DNA antibodies are highly specific for SLE and are found in approximately 60-70% of patients with the disease. Higher antibody titers are associated with more active disease and can help guide treatment decisions. It is important to note that this test is not used as a standalone diagnostic tool for SLE, as the presence of anti-ds DNA antibodies can also be seen in other autoimmune diseases and even some infectious diseases. It is usually combined with other clinical and laboratory findings to make a definitive diagnosis. |
