Immunohistochemistry CD8 Test sale cost 410 AED
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Immunohistochemistry CD8 Test Cost

Original price was: 460 د.إ.Current price is: 410 د.إ.

-11%

The Immunohistochemistry CD8 Test is a specialized diagnostic procedure designed to detect and quantify CD8-positive cells within tissue samples. CD8 is a marker commonly found on the surface of cytotoxic T cells, which play a crucial role in the immune system by directly killing infected or cancerous cells. By applying specific antibodies that bind to CD8 molecules, this test can highlight the presence and distribution of these immune cells within a tissue section, providing valuable insights into the immune response within a particular area of the body.

This test is particularly useful in the context of cancer diagnosis and research, as it can help in assessing the immune response to tumors and potentially guide immunotherapy treatments. It is also utilized in studying infectious diseases and autoimmune conditions, where the behavior of CD8-positive cells can be a critical factor in disease progression and response to treatment.

In the UAE, the Immunohistochemistry CD8 Test is available at DNA Labs, a reputable laboratory known for its advanced diagnostic services. The cost of the test is 410 AED, making it accessible for both healthcare professionals and patients seeking in-depth analysis of immune-related conditions. DNA Labs UAE is equipped with state-of-the-art technology and staffed by experienced professionals, ensuring accurate and reliable test results.

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IMMUNOHISTOCHEMISTRY CD8 Test

Cost: AED 410.0

Test Name: IMMUNOHISTOCHEMISTRY CD8 Test

Components: CD8 protein

Price: 410.0 AED

Sample Condition: Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Report Delivery: Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex: 7 days

Method: Immunohistochemistry

Test Type: Cancer

Doctor: Oncologist, Pathologist

Test Department: DNA Labs UAE

Pre Test Information: Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Test Details

CD8 is a protein found on the surface of cytotoxic T cells, which are a type of immune cell involved in killing infected or abnormal cells. Immunohistochemistry (IHC) is a technique used to visualize and detect specific proteins in tissue samples.

To perform an immunohistochemistry CD8 test, the following steps are typically involved:

  1. Tissue preparation: The tissue sample, usually obtained through a biopsy or surgical procedure, is fixed in formalin and embedded in paraffin wax. The sample is then cut into thin sections (around 4-6 micrometers thick) using a microtome.
  2. Deparaffinization and rehydration: The paraffin wax is removed from the tissue sections by immersing them in xylene or other clearing agents. The sections are then rehydrated through a series of alcohol solutions of decreasing concentration.
  3. Antigen retrieval: The tissue sections are subjected to antigen retrieval techniques to unmask the target protein and improve antibody binding. This can be done through heat-induced epitope retrieval (HIER) or enzymatic digestion.
  4. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking agent, such as bovine serum albumin (BSA) or normal serum, to prevent false positive results.
  5. Primary antibody incubation: The tissue sections are incubated with a primary antibody specific to CD8. The primary antibody binds to the CD8 protein if it is present in the tissue sample.
  6. Secondary antibody incubation: After washing off any unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system, such as a fluorescent dye or an enzyme. The secondary antibody binds to the primary antibody, amplifying the signal and making it detectable.
  7. Visualization: If an enzyme-based detection system is used, a chromogenic substrate is added, resulting in the formation of a visible color at the site of CD8 protein expression. If a fluorescent dye is used, the tissue sections are directly observed under a fluorescence microscope.
  8. Counterstaining and mounting: To enhance the contrast and visualization of the tissue structures, the sections may be counterstained with dyes like hematoxylin. Finally, the sections are mounted on glass slides using a mounting medium and covered with a coverslip.
  9. Analysis: The stained tissue sections are then examined under a microscope by a pathologist or researcher to determine the presence and distribution of CD8-positive cells.

The immunohistochemistry CD8 test allows for the visualization and quantification of CD8-positive immune cells in tissue samples, providing valuable information about the immune response and potential immune-related diseases.

Test Name IMMUNOHISTOCHEMISTRY CD8 Test
Components
Price 410.0 AED
Sample Condition Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Report Delivery Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days
Method Immunohistochemistry
Test type Cancer
Doctor Oncologist, Pathologist
Test Department:
Pre Test Information Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Test Details

CD8 is a protein found on the surface of cytotoxic T cells, which are a type of immune cell involved in killing infected or abnormal cells. Immunohistochemistry (IHC) is a technique used to visualize and detect specific proteins in tissue samples.

To perform an immunohistochemistry CD8 test, the following steps are typically involved:

1. Tissue preparation: The tissue sample, usually obtained through a biopsy or surgical procedure, is fixed in formalin and embedded in paraffin wax. The sample is then cut into thin sections (around 4-6 micrometers thick) using a microtome.

2. Deparaffinization and rehydration: The paraffin wax is removed from the tissue sections by immersing them in xylene or other clearing agents. The sections are then rehydrated through a series of alcohol solutions of decreasing concentration.

3. Antigen retrieval: The tissue sections are subjected to antigen retrieval techniques to unmask the target protein and improve antibody binding. This can be done through heat-induced epitope retrieval (HIER) or enzymatic digestion.

4. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking agent, such as bovine serum albumin (BSA) or normal serum, to prevent false positive results.

5. Primary antibody incubation: The tissue sections are incubated with a primary antibody specific to CD8. The primary antibody binds to the CD8 protein if it is present in the tissue sample.

6. Secondary antibody incubation: After washing off any unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system, such as a fluorescent dye or an enzyme. The secondary antibody binds to the primary antibody, amplifying the signal and making it detectable.

7. Visualization: If an enzyme-based detection system is used, a chromogenic substrate is added, resulting in the formation of a visible color at the site of CD8 protein expression. If a fluorescent dye is used, the tissue sections are directly observed under a fluorescence microscope.

8. Counterstaining and mounting: To enhance the contrast and visualization of the tissue structures, the sections may be counterstained with dyes like hematoxylin. Finally, the sections are mounted on glass slides using a mounting medium and covered with a coverslip.

9. Analysis: The stained tissue sections are then examined under a microscope by a pathologist or researcher to determine the presence and distribution of CD8-positive cells.

The immunohistochemistry CD8 test allows for the visualization and quantification of CD8-positive immune cells in tissue samples, providing valuable information about the immune response and potential immune-related diseases.

SKU: TEST-6497 Category:

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