VITAMIN C ASCORBIC ACID Test
At DNA Labs UAE, we offer the VITAMIN C ASCORBIC ACID Test to help determine the concentration of ascorbic acid (Vitamin C) in a solution. This colorimetric test relies on the reduction of a colored dye by ascorbic acid.
Test Details
Test Name: VITAMIN C ASCORBIC ACID Test
Components: 2 mL (1 mL min.) serum from 1 SST. Separate serum within 3 hours of collection in a plastic transport vial and freeze. Ship frozen. DO NOT THAW. Wrap in aluminium foil to protect from light. Avoid vitamin supplements & fruits 24 hours prior to sample collection. Overnight fasting is preferred. Duly filled Test Send Out Consent Form is mandatory.
Price: 1540.0 AED
Report Delivery: Sample by 7th of the month; Report after 2-3 weeks
Method: Chromatography, Mass Spectrometry
Test Type: Nutritional disorders
Doctor: Physician
Test Department: OS
Pre Test Information: Avoid vitamin supplements & fruits 24 hours prior to sample collection. Overnight fasting is preferred. Wrap sample in aluminium foil to protect from light. Duly filled Test Send Out Consent Form is mandatory.
Test Procedure
- Prepare a series of test tubes or cuvettes, each containing different volumes of the solution to be tested for Vitamin C. Label them accordingly.
- Add a fixed volume of a standardized solution of ascorbic acid to a separate test tube or cuvette. This will serve as the reference solution.
- Prepare a reagent solution by dissolving a specific dye, such as 2,6-dichlorophenolindophenol (DCPIP), in a suitable solvent. The concentration of the dye should be known.
- Add a few drops of the reagent solution to each test tube or cuvette, including the reference solution. Mix gently.
- Observe the color change in each solution. Initially, the dye will have a specific color, and ascorbic acid will reduce the dye, causing the color to fade.
- Compare the intensity of color change in each test tube or cuvette to the reference solution. The greater the concentration of ascorbic acid, the more intense the color change will be.
- Measure the absorbance of each solution at a specific wavelength using a spectrophotometer. This will provide quantitative data for the concentration of ascorbic acid in each solution.
- Plot a calibration curve using the absorbance values obtained from the reference solution and known concentrations of ascorbic acid. This curve can be used to determine the concentration of ascorbic acid in the test solutions.
It is important to note that the VITAMIN C ASCORBIC ACID Test is a qualitative and quantitative method for determining the concentration of ascorbic acid. It is commonly used in food and pharmaceutical industries to ensure the accuracy of Vitamin C content in various products.
| Test Name | VITAMIN C ASCORBIC ACID Test |
|---|---|
| Components | |
| Price | 1540.0 AED |
| Sample Condition | 2 mL (1 mL min.) serum from 1 SST. Separateserumwithin3hoursof collectionina plastictransportvialand freeze. Shipfrozen. DONOT THAW. Wrapinaluminiumfoiltoprotectfrom light.Avoidvitaminsupplements& fruits24hourspriortosample collection.Overnightfastingis preferred. Duly filled Test Send Out Consent Formis mandatory. |
| Report Delivery | Sampleby7thofthemonth;Report after2??3weeks |
| Method | Chromatography, Mass Spectrometry |
| Test type | Nutritional disorders |
| Doctor | Physician |
| Test Department: | OS |
| Pre Test Information | Avoidvitaminsupplements& fruits24hours priortosample collection.Overnightfastingis preferred. Wrap sample inaluminiumfoilto protectfromlight. Duly filled Test Send Out Consent Formis mandatory. |
| Test Details |
The Vitamin C Ascorbic Acid Test is a method used to determine the concentration of ascorbic acid (Vitamin C) in a solution. It is a colorimetric test that relies on the reduction of a colored dye by ascorbic acid. Here is a step-by-step procedure for the test: 1. Prepare a series of test tubes or cuvettes, each containing different volumes of the solution to be tested for Vitamin C. Label them accordingly. 2. Add a fixed volume of a standardized solution of ascorbic acid to a separate test tube or cuvette. This will serve as the reference solution. 3. Prepare a reagent solution by dissolving a specific dye, such as 2,6-dichlorophenolindophenol (DCPIP), in a suitable solvent. The concentration of the dye should be known. 4. Add a few drops of the reagent solution to each test tube or cuvette, including the reference solution. Mix gently. 5. Observe the color change in each solution. Initially, the dye will have a specific color, and ascorbic acid will reduce the dye, causing the color to fade. 6. Compare the intensity of color change in each test tube or cuvette to the reference solution. The greater the concentration of ascorbic acid, the more intense the color change will be. 7. Measure the absorbance of each solution at a specific wavelength using a spectrophotometer. This will provide quantitative data for the concentration of ascorbic acid in each solution. 8. Plot a calibration curve using the absorbance values obtained from the reference solution and known concentrations of ascorbic acid. This curve can be used to determine the concentration of ascorbic acid in the test solutions. It is important to note that the Vitamin C Ascorbic Acid Test is a qualitative and quantitative method for determining the concentration of ascorbic acid. It is commonly used in food and pharmaceutical industries to ensure the accuracy of Vitamin C content in various products. |
