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Shigella Dysenterica RNA Detection Qualitative Test

950 د.إ

-15%

The Shigella Dysenterica RNA Detection Qualitative Test is a specialized diagnostic procedure designed to identify the presence of Shigella dysenteriae, the bacterium responsible for causing dysentery, particularly in regions with poor sanitation. This test specifically targets the RNA sequences unique to the Shigella dysenteriae pathogen, employing sophisticated molecular techniques to ascertain the presence or absence of the bacterium’s genetic material in a patient’s sample. This qualitative approach offers a high degree of specificity and sensitivity, making it an invaluable tool in the timely diagnosis and management of dysentery outbreaks.

Performed at DNA Labs UAE, a leading facility known for its advanced diagnostic technologies and expert staff, this test ensures reliable results. The cost of the test is set at 950 AED, reflecting the sophisticated nature of the testing methodology and the expertise required to interpret the results. By opting for this test, healthcare providers can make informed decisions regarding the treatment and control of dysentery, ultimately contributing to improved patient outcomes and public health.

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Shigella Dysentrica RNA Detection Qualitative Test

Introduction

Welcome to DNA Labs UAE, where we offer the Shigella Dysentrica RNA Detection Qualitative Test. This test is designed to detect the presence of Shigella dysenteriae-specific RNA sequences in a sample using molecular techniques such as polymerase chain reaction (PCR) or nucleic acid amplification tests (NAATs).

Test Details

The qualitative test for detecting Shigella dysenteriae RNA involves several steps:

  1. Sample collection: A sample is collected from the patient suspected of having a Shigella dysenteriae infection. This sample can be stool, rectal swab, or any other appropriate specimen.
  2. Nucleic acid extraction: The RNA is extracted from the collected sample using specialized extraction kits or methods. This step is crucial to obtain pure RNA for further analysis.
  3. Reverse transcription: The extracted RNA is reverse transcribed into complementary DNA (cDNA) using reverse transcriptase enzyme. This step converts the RNA into a more stable and amplifiable form.
  4. PCR amplification: The cDNA is then subjected to PCR using specific primers that target Shigella dysenteriae RNA sequences. These primers are designed to amplify only the target RNA sequences if present in the sample.
  5. Gel electrophoresis: The PCR products are separated by gel electrophoresis, which helps visualize the amplified DNA fragments. If the target RNA sequences were present in the sample, a band of the expected size should be visible on the gel.
  6. Interpretation: The presence or absence of the target band on the gel determines the qualitative result. If the band is present, it indicates the presence of Shigella dysenteriae RNA in the sample, suggesting an active infection. If the band is absent, it indicates the absence of Shigella dysenteriae RNA or a very low level of the target RNA in the sample.

Test Name

Shigella Dysentrica RNA Detection Qualitative Test

Components

Price: 950.0 AED

Sample Condition

Whole Blood, rectal swabs, food samples, water sample

Report Delivery

3rd Working Day Email: 36 hours. On phone: 24 hours

Method

Real Time PCR

Test Type

Viral

Doctor

Physician

Test Department

Genetics

Pre Test Information

Need to sign Consent document and bring any clinical history of patient for Shigella Dysentrica (RNA Detection) Qualitative Test

It is important to note that this is a general outline, and the specific details of the test may vary depending on the laboratory and the specific RNA detection method used. Additionally, it is always recommended to consult with a healthcare professional or a laboratory specialist for accurate and reliable diagnosis.

Test Name Shigella Dysentrica RNA Detection Qualitative Test
Components
Price 950.0 AED
Sample Condition Whole Blood, rectal swabs, food samples, water sample
Report Delivery 3rd Working Day Email:-36 hours. On phone: 24 hours
Method Real Time PCR
Test type Viral
Doctor Physician
Test Department: Genetics
Pre Test Information Need to sign Consent document and bring any clinical history of patient forShigella Dysentrica (RNA Detection) QualitativeTest
Test Details

The qualitative test for detecting Shigella dysenteriae RNA involves the use of molecular techniques such as polymerase chain reaction (PCR) or nucleic acid amplification tests (NAATs). These tests are designed to detect the presence of Shigella dysenteriae-specific RNA sequences in a sample.

Here is a general outline of the steps involved in the qualitative RNA detection test:

1. Sample collection: A sample is collected from the patient suspected of having a Shigella dysenteriae infection. This sample can be stool, rectal swab, or any other appropriate specimen.

2. Nucleic acid extraction: The RNA is extracted from the collected sample using specialized extraction kits or methods. This step is crucial to obtain pure RNA for further analysis.

3. Reverse transcription: The extracted RNA is reverse transcribed into complementary DNA (cDNA) using reverse transcriptase enzyme. This step converts the RNA into a more stable and amplifiable form.

4. PCR amplification: The cDNA is then subjected to PCR using specific primers that target Shigella dysenteriae RNA sequences. These primers are designed to amplify only the target RNA sequences if present in the sample.

5. Gel electrophoresis: The PCR products are separated by gel electrophoresis, which helps visualize the amplified DNA fragments. If the target RNA sequences were present in the sample, a band of the expected size should be visible on the gel.

6. Interpretation: The presence or absence of the target band on the gel determines the qualitative result. If the band is present, it indicates the presence of Shigella dysenteriae RNA in the sample, suggesting an active infection. If the band is absent, it indicates the absence of Shigella dysenteriae RNA or a very low level of the target RNA in the sample.

It is important to note that this is a general outline, and the specific details of the test may vary depending on the laboratory and the specific RNA detection method used. Additionally, it is always recommended to consult with a healthcare professional or a laboratory specialist for accurate and reliable diagnosis.