PNH Comprehensive Work Up Flowcytometry-Flaer CD14 CD15 CD24 CD45CD59 CD64 GlY-A Test
At DNA Labs UAE, we offer the PNH Comprehensive Work Up Flowcytometry-Flaer CD14 CD15 CD24 CD45CD59 CD64 GlY-A Test. This test is an important diagnostic tool for identifying and monitoring paroxysmal nocturnal hemoglobinuria (PNH), a rare acquired hematopoietic stem cell disorder.
Test Components
The PNH Comprehensive Work Up Flowcytometry-Flaer CD14 CD15 CD24 CD45CD59 CD64 GlY-A Test includes:
- EDTA Vacutainer (2ml) or Sodium heparin Vacutainer (2ml)
Price
The cost of the PNH Comprehensive Work Up Flowcytometry-Flaer CD14 CD15 CD24 CD45CD59 CD64 GlY-A Test is 2400.0 AED.
Sample Condition
The test requires a peripheral blood sample.
Report Delivery
You can expect to receive your test report within 1-2 days.
Method
The PNH Comprehensive Work Up Flowcytometry-Flaer CD14 CD15 CD24 CD45CD59 CD64 GlY-A Test is conducted using flow cytometry, a widely used technique for diagnosing PNH.
Test Type
The PNH Comprehensive Work Up Flowcytometry-Flaer CD14 CD15 CD24 CD45CD59 CD64 GlY-A Test falls under the category of genetics tests.
Doctor
The test can be ordered by a General Surgeon.
Pre Test Information
It is important to note that the PNH Comprehensive Work Up(Flowcytometry)-Flaer, CD14, CD15, CD24, CD45, CD59, CD64, GlY-A Test requires a doctor’s prescription. However, prescriptions are not applicable for surgery and pregnancy cases, as well as individuals planning to travel abroad.
Test Details
PNH (paroxysmal nocturnal hemoglobinuria) is a rare acquired hematopoietic stem cell disorder characterized by the abnormal expansion of a clone of cells that lack certain proteins on their surface. This results in the destruction of red blood cells (hemolysis), leading to symptoms such as hemoglobinuria (dark urine), anemia, fatigue, and thrombosis.
Flow cytometry is a technique commonly used to diagnose PNH and assess the size of the PNH clone. It involves the use of fluorescently-labeled antibodies that bind to specific cell surface markers. By analyzing the fluorescence pattern of individual cells, flow cytometry can identify abnormal populations of cells in the blood.
The comprehensive work-up for PNH using flow cytometry typically includes the following markers:
- FLAER (fluorescent aerolysin): FLAER is a toxin derived from the bacteria Aeromonas hydrophila that specifically binds to glycosylphosphatidylinositol (GPI) anchors. In PNH, the absence of GPI-anchored proteins on the cell surface can be detected using FLAER, which binds to the residual GPI anchors on PNH cells.
- CD14: CD14 is a marker expressed on monocytes and macrophages. In PNH, the absence of CD14 on the cell surface can indicate the presence of PNH clones.
- CD15: CD15 is a marker expressed on neutrophils and some monocytes. The absence of CD15 on the cell surface can be indicative of PNH clones.
- CD24: CD24 is a marker expressed on lymphocytes, monocytes, and granulocytes. The absence of CD24 on the cell surface can suggest the presence of PNH clones.
- CD45: CD45 is a marker expressed on all nucleated hematopoietic cells. PNH clones often have decreased expression of CD45, which can be detected using flow cytometry.
- CD59: CD59 is a GPI-anchored protein that inhibits the formation of the membrane attack complex (MAC), which is responsible for the destruction of red blood cells. The absence of CD59 on the cell surface is a hallmark of PNH.
- CD64: CD64 is a marker expressed on monocytes and macrophages. Its absence on the cell surface can indicate the presence of PNH clones.
- Gly-A: Gly-A is a marker expressed on red blood cells. The absence of Gly-A on the cell surface can suggest the presence of PNH clones.
By analyzing the expression levels of these markers on different cell populations, flow cytometry can provide valuable information about the presence and size of PNH clones. This information is crucial for diagnosing PNH and monitoring disease progression.
| Test Name | PNH Comprehensive Work Up Flowcytometry-Flaer CD14 CD15 CD24 CD45CD59 CD64 GlY-A Test |
|---|---|
| Components | EDTA Vacutainer (2ml)/Sodium heparin Vacutainer (2ml) |
| Price | 2400.0 AED |
| Sample Condition | Peripheral blood |
| Report Delivery | 1-2 days |
| Method | Flowcytometry |
| Test type | Genetics |
| Doctor | General Surgeon |
| Test Department: | |
| Pre Test Information | PNH Comprehensive Work Up(Flowcytometry)-Flaer, CD14, CD15, CD24, CD45,CD59, CD64, GlY-A can be done with a Doctors prescription. Prescription is not applicable for surgery and pregnancy cases or people planing to travel abroad. |
| Test Details | PNH (paroxysmal nocturnal hemoglobinuria) is a rare acquired hematopoietic stem cell disorder characterized by the abnormal expansion of a clone of cells that lack certain proteins on their surface. This results in the destruction of red blood cells (hemolysis), leading to symptoms such as hemoglobinuria (dark urine), anemia, fatigue, and thrombosis. Flow cytometry is a technique commonly used to diagnose PNH and assess the size of the PNH clone. It involves the use of fluorescently-labeled antibodies that bind to specific cell surface markers. By analyzing the fluorescence pattern of individual cells, flow cytometry can identify abnormal populations of cells in the blood. The comprehensive work-up for PNH using flow cytometry typically includes the following markers: 1. FLAER (fluorescent aerolysin): FLAER is a toxin derived from the bacteria Aeromonas hydrophila that specifically binds to glycosylphosphatidylinositol (GPI) anchors. In PNH, the absence of GPI-anchored proteins on the cell surface can be detected using FLAER, which binds to the residual GPI anchors on PNH cells. 2. CD14: CD14 is a marker expressed on monocytes and macrophages. In PNH, the absence of CD14 on the cell surface can indicate the presence of PNH clones. 3. CD15: CD15 is a marker expressed on neutrophils and some monocytes. The absence of CD15 on the cell surface can be indicative of PNH clones. 4. CD24: CD24 is a marker expressed on lymphocytes, monocytes, and granulocytes. The absence of CD24 on the cell surface can suggest the presence of PNH clones. 5. CD45: CD45 is a marker expressed on all nucleated hematopoietic cells. PNH clones often have decreased expression of CD45, which can be detected using flow cytometry. 6. CD59: CD59 is a GPI-anchored protein that inhibits the formation of the membrane attack complex (MAC), which is responsible for the destruction of red blood cells. The absence of CD59 on the cell surface is a hallmark of PNH. 7. CD64: CD64 is a marker expressed on monocytes and macrophages. Its absence on the cell surface can indicate the presence of PNH clones. 8. Gly-A: Gly-A is a marker expressed on red blood cells. The absence of Gly-A on the cell surface can suggest the presence of PNH clones. By analyzing the expression levels of these markers on different cell populations, flow cytometry can provide valuable information about the presence and size of PNH clones. This information is crucial for diagnosing PNH and monitoring disease progression. |
