IMMUNOPHENOTYPING BY FLOW CYTOMETRY IgM HEAVY CHAIN SURFACE Test
Test Cost: AED 480.0
Test Details
Immunophenotyping by flow cytometry is a technique used to identify and characterize different cell populations based on the expression of specific cell surface markers. In the case of the IgM heavy chain surface test, the goal is to detect and quantify the expression of IgM heavy chain on the surface of cells.
IgM is an immunoglobulin isotype that is primarily involved in the early immune response against pathogens. It is the first antibody produced during an immune response and is found on the surface of B cells as a membrane-bound form.
To perform the IgM heavy chain surface test, cells of interest, typically B cells, are isolated from a sample, such as blood or tissue. These cells are then stained with fluorescently labeled antibodies specific to the IgM heavy chain. The antibodies will bind to the IgM heavy chain on the surface of the cells, allowing for their detection and quantification using flow cytometry.
Flow cytometry is a technique that utilizes lasers to analyze individual cells as they pass through a flow cell. The fluorescently labeled cells are excited by the laser, and the emitted fluorescence is detected by photodetectors. This information is then processed and analyzed to determine the presence and level of IgM heavy chain expression on the cell surface.
The IgM heavy chain surface test can provide valuable information about the activation and differentiation status of B cells, as well as their involvement in immune responses. It can be used in research settings to study immune disorders, monitor immune responses to infections or vaccines, and evaluate the efficacy of immunotherapies.
Test Components and Price
Test Name: IMMUNOPHENOTYPING BY FLOW CYTOMETRY IgM HEAVY CHAIN SURFACE Test
Components: 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube and 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube OR 2 mL (1 mL min.) Bone marrow in 1 Green Top (Sodium heparin) tube
Price: 480.0 AED
Sample Condition and Report Delivery
Ship the sample immediately at 18-22°C. DO NOT REFRIGERATE OR FREEZE. Specify time, date, and clinical details on the test request form.
Report Delivery: Sample daily by 9 am; Report same day
Test Type and Doctor
Test Type: Cancer
Doctor: Oncologist, Hematologist
Test Department
Test Department: FLOW CYTOMETRY
Pre Test Information
Give brief clinical history.
Conclusion
Overall, immunophenotyping by flow cytometry of IgM heavy chain surface expression is a powerful tool for understanding the role of B cells in the immune system and can have important clinical applications in diagnosing and monitoring immune-related diseases.
| Test Name | IMMUNOPHENOTYPING BY FLOW CYTOMETRY IgM HEAVY CHAIN SURFACE Test |
|---|---|
| Components | |
| Price | 480.0 AED |
| Sample Condition | 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tubeAND 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube OR 2 mL (1 mL min.) Bone marrow in 1 Green Top (Sodium heparin) tube. Ship immediately at 18\u0192??22?\u00f8C. DO NOT REFRIGERATE OR FREEZE. Specify time, date and clinical details on test request form. |
| Report Delivery | Sample Daily by 9 am; Report Same day |
| Method | Flow Cytometry |
| Test type | Cancer |
| Doctor | Oncologist, Hematologist |
| Test Department: | FLOW CYTOMETRY |
| Pre Test Information | Give brief clinical history. |
| Test Details |
Immunophenotyping by flow cytometry is a technique used to identify and characterize different cell populations based on the expression of specific cell surface markers. In the case of IgM heavy chain surface test, the goal is to detect and quantify the expression of IgM heavy chain on the surface of cells. IgM is an immunoglobulin isotype that is primarily involved in the early immune response against pathogens. It is the first antibody produced during an immune response and is found on the surface of B cells as a membrane-bound form. To perform the IgM heavy chain surface test, cells of interest, typically B cells, are isolated from a sample, such as blood or tissue. These cells are then stained with fluorescently labeled antibodies specific to the IgM heavy chain. The antibodies will bind to the IgM heavy chain on the surface of the cells, allowing for their detection and quantification using flow cytometry. Flow cytometry is a technique that utilizes lasers to analyze individual cells as they pass through a flow cell. The fluorescently labeled cells are excited by the laser, and the emitted fluorescence is detected by photodetectors. This information is then processed and analyzed to determine the presence and level of IgM heavy chain expression on the cell surface. The IgM heavy chain surface test can provide valuable information about the activation and differentiation status of B cells, as well as their involvement in immune responses. It can be used in research settings to study immune disorders, monitor immune responses to infections or vaccines, and evaluate the efficacy of immunotherapies. Overall, immunophenotyping by flow cytometry of IgM heavy chain surface expression is a powerful tool for understanding the role of B cells in the immune system and can have important clinical applications in diagnosing and monitoring immune-related diseases. |
