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Immunophenotyping by Flow Cytometry CD14 Test

Original price was: 530 د.إ.Current price is: 480 د.إ.

-9%

Immunophenotyping by Flow Cytometry CD14 Test is a sophisticated diagnostic procedure utilized to analyze the expression of the CD14 molecule on the surface of cells, particularly monocytes and macrophages, in a blood or bone marrow sample. This test is pivotal in the study of immune system disorders, infections, and certain types of leukemia, where the presence and quantity of CD14-expressing cells can provide critical insights into the patient’s health status and guide treatment decisions.

Flow cytometry, the technology behind this test, allows for the rapid and accurate identification of cell populations by their physical and chemical characteristics. The CD14 test is especially important in the context of diagnosing and monitoring diseases that affect the immune system, as CD14 plays a key role in the innate immune response and inflammation.

In the United Arab Emirates, this specific test is available at DNA Labs UAE, a reputable facility known for its state-of-the-art diagnostic services. The cost of the Immunophenotyping by Flow Cytometry CD14 Test at DNA Labs UAE is 480 AED. This price reflects the intricate technology and expertise required to conduct the test and analyze its results, ensuring patients receive accurate and actionable health information.

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IMMUNOPHENOTYPING BY FLOW CYTOMETRY CD14 Test

Test Name: IMMUNOPHENOTYPING BY FLOW CYTOMETRY CD14 Test

Components: CD14 Test

Price: 480.0 AED

Sample Condition: 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube and 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube OR 2 mL (1 mL min.) Bone marrow in 1 Green Top (Sodium heparin) tube. Ship immediately at 18-22°C. DO NOT REFRIGERATE OR FREEZE. Specify time, date, and clinical details on the test request form.

Report Delivery: Sample Daily by 9 am; Report Same day

Method: Flow Cytometry

Test type: Cancer

Doctor: Oncologist, Hematologist

Test Department: FLOW CYTOMETRY

Pre Test Information: Give brief clinical history.

Test Details:

CD14 is a surface marker protein that is primarily expressed on the surface of monocytes and macrophages. It plays a role in the innate immune response and acts as a receptor for lipopolysaccharide (LPS), a component of bacterial cell walls.

Immunophenotyping by flow cytometry is a technique used to analyze and quantify the expression of specific surface markers on cells. In the case of CD14, flow cytometry can be used to determine the percentage of cells expressing this marker within a population of cells.

To perform a CD14 immunophenotyping test, the following steps are typically followed:

  1. Sample preparation: Blood or tissue samples are collected from the patient and processed to obtain a single-cell suspension.
  2. Antibody staining: Fluorescently-labeled antibodies specific for CD14 are added to the cell suspension. These antibodies will bind to CD14 if it is present on the cell surface.
  3. Incubation: The cell suspension is incubated for a period of time to allow the antibodies to bind to the CD14 protein.
  4. Flow cytometry analysis: The cell suspension is passed through a flow cytometer, which uses lasers to excite the fluorescent labels on the antibodies. The emitted fluorescence is then detected and measured by the flow cytometer.
  5. Data analysis: The flow cytometry data is analyzed using specialized software to determine the percentage of cells expressing CD14. This can be further analyzed in combination with other markers to identify specific cell populations within the sample.

CD14 immunophenotyping by flow cytometry is commonly used in research and clinical settings to study immune cell populations and assess immune system function. It can be particularly useful in diagnosing and monitoring conditions such as sepsis, autoimmune disorders, and certain types of cancer.

Test Name IMMUNOPHENOTYPING BY FLOW CYTOMETRY CD14 Test
Components
Price 480.0 AED
Sample Condition 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tubeAND 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube OR 2 mL (1 mL min.) Bone marrow in 1 Green Top (Sodium heparin) tube. Ship immediately at 18\u0192??22?\u00f8C. DO NOT REFRIGERATE OR FREEZE. Specify time, date and clinical details on test request form.
Report Delivery Sample Daily by 9 am; Report Same day
Method Flow Cytometry
Test type Cancer
Doctor Oncologist, Hematologist
Test Department: FLOW CYTOMETRY
Pre Test Information Give brief clinical history.
Test Details

CD14 is a surface marker protein that is primarily expressed on the surface of monocytes and macrophages. It plays a role in the innate immune response and acts as a receptor for lipopolysaccharide (LPS), a component of bacterial cell walls.

Immunophenotyping by flow cytometry is a technique used to analyze and quantify the expression of specific surface markers on cells. In the case of CD14, flow cytometry can be used to determine the percentage of cells expressing this marker within a population of cells.

To perform a CD14 immunophenotyping test, the following steps are typically followed:

1. Sample preparation: Blood or tissue samples are collected from the patient and processed to obtain a single-cell suspension.

2. Antibody staining: Fluorescently-labeled antibodies specific for CD14 are added to the cell suspension. These antibodies will bind to CD14 if it is present on the cell surface.

3. Incubation: The cell suspension is incubated for a period of time to allow the antibodies to bind to the CD14 protein.

4. Flow cytometry analysis: The cell suspension is passed through a flow cytometer, which uses lasers to excite the fluorescent labels on the antibodies. The emitted fluorescence is then detected and measured by the flow cytometer.

5. Data analysis: The flow cytometry data is analyzed using specialized software to determine the percentage of cells expressing CD14. This can be further analyzed in combination with other markers to identify specific cell populations within the sample.

CD14 immunophenotyping by flow cytometry is commonly used in research and clinical settings to study immune cell populations and assess immune system function. It can be particularly useful in diagnosing and monitoring conditions such as sepsis, autoimmune disorders, and certain types of cancer.