IMMUNOHISTOCHEMISTRY SYNAPTOPHYSIN Test
Test Name: IMMUNOHISTOCHEMISTRY SYNAPTOPHYSIN Test
Components: Synaptophysin protein detection
Price: 410.0 AED
Sample Condition: Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Report Delivery: Sample Daily by 6 pm; Report Block: 5 days, Tissue Biopsy: 5 days, Tissue large complex: 7 days
Method: Immunohistochemistry
Test Type: Cancer
Doctor: Oncologist, Pathologist
Test Department: DNA Labs UAE
Pre Test Information: Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Test Details
Immunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples. The synaptophysin test is used to identify and quantify the presence of synaptophysin in tissue samples, particularly in the brain. This test is commonly used in neuropathology to diagnose and classify neuroendocrine tumors and evaluate neuronal differentiation in various types of brain tumors.
To perform the synaptophysin test, tissue samples are fixed, embedded in paraffin, and sectioned into thin slices. These tissue sections are then incubated with a primary antibody specific to synaptophysin. The primary antibody binds to the synaptophysin protein if it is present in the tissue. After washing off the unbound primary antibody, a secondary antibody is applied, which is conjugated to a detection molecule, such as an enzyme or a fluorophore. This secondary antibody amplifies the signal. Finally, a chromogen or fluorescent substrate is added, which produces a visible color or fluorescence in the presence of the detection molecule, indicating the presence of synaptophysin.
The stained tissue sections are then examined under a microscope, and the presence and distribution of synaptophysin in the tissue can be assessed. The intensity and localization of the staining provide information about the functionality and organization of synapses in the tissue.
The synaptophysin test is a valuable tool in diagnosing and studying various neurological conditions, as it allows for the visualization and quantification of synapses in tissue samples. It provides important information about the presence and distribution of synaptophysin, which can aid in understanding the underlying mechanisms of neuronal function and dysfunction.
Test Name | IMMUNOHISTOCHEMISTRY SYNAPTOPHYSIN Test |
---|---|
Components | |
Price | 410.0 AED |
Sample Condition | Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history. |
Report Delivery | Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days |
Method | Immunohistochemistry |
Test type | Cancer |
Doctor | Oncologist, Pathologist |
Test Department: | |
Pre Test Information | Provide a copy of the Histopathology report, Site of biopsy and Clinical history. |
Test Details |
Immunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples. Synaptophysin is a protein found in synaptic vesicles, which are responsible for the release of neurotransmitters in neuronal synapses. The synaptophysin test is used to identify and quantify the presence of synaptophysin in tissue samples, particularly in the brain. The synaptophysin test is commonly used in neuropathology to diagnose and classify neuroendocrine tumors, as well as to evaluate neuronal differentiation in various types of brain tumors. It can also be used to study synaptic plasticity and neuronal development in research settings. To perform the synaptophysin test, tissue samples are fixed, embedded in paraffin, and sectioned into thin slices. These tissue sections are then incubated with a primary antibody specific to synaptophysin. The primary antibody binds to the synaptophysin protein if it is present in the tissue. After washing off the unbound primary antibody, a secondary antibody is applied, which is conjugated to a detection molecule, such as an enzyme or a fluorophore. This secondary antibody binds to the primary antibody, amplifying the signal. Finally, a chromogen or fluorescent substrate is added, which produces a visible color or fluorescence in the presence of the detection molecule, indicating the presence of synaptophysin. The stained tissue sections are then examined under a microscope, and the presence and distribution of synaptophysin in the tissue can be assessed. The intensity and localization of the staining can provide information about the functionality and organization of synapses in the tissue. The synaptophysin test is a valuable tool in diagnosing and studying various neurological conditions, as it allows for the visualization and quantification of synapses in tissue samples. It provides important information about the presence and distribution of synaptophysin, which can aid in understanding the underlying mechanisms of neuronal function and dysfunction. |