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Immunohistochemistry Prolactin Test

410 د.إ

-11%

The Immunohistochemistry Prolactin Test is a specialized diagnostic procedure utilized to measure the levels of prolactin hormone in the body. Prolactin is a hormone produced by the pituitary gland, which plays a crucial role in lactation and fertility. Abnormal levels of prolactin can lead to various health issues, including infertility, menstrual irregularities, and pituitary tumors.

This test employs immunohistochemistry (IHC), a technique that combines immunology, histochemistry, and microscopy to detect specific antigens in cells within a tissue section. The method uses antibodies coated with a dye to visualize the presence and quantity of prolactin in tissue samples, providing valuable insights into the hormone’s role and its pathological excess or deficiency in the body.

At DNA Labs UAE, the Immunohistochemistry Prolactin Test is offered at a cost of 410 AED. DNA Labs UAE is renowned for its state-of-the-art facilities and expertise in providing accurate and reliable diagnostic services. Conducting the test in such a reputable laboratory ensures high-quality results, which are crucial for the accurate diagnosis and treatment planning of conditions related to prolactin imbalance.

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IMMUNOHISTOCHEMISTRY PROLACTIN Test

Test Name: IMMUNOHISTOCHEMISTRY PROLACTIN Test

Components: Prolactin Test

Price: 410.0 AED

Sample Condition: Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Report Delivery: Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex: 7 days

Method: Immunohistochemistry

Test type: Cancer

Doctor: Oncologist, Pathologist

Test Department: Pre Test Information

Pre Test Information: Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Test Details:

Immunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples. In the case of the prolactin test, IHC can be used to identify the presence and distribution of prolactin in a tissue sample. To perform an immunohistochemistry prolactin test, the following steps are typically followed:

  1. Tissue fixation: The tissue sample is collected and fixed using a suitable fixative, such as formalin. This helps preserve the tissue structure and proteins.
  2. Tissue processing: The fixed tissue is then processed by embedding it in paraffin wax, which allows for easier handling and sectioning.
  3. Sectioning: Thin sections (usually around 4-6 micrometers) of the paraffin-embedded tissue are cut using a microtome. These sections are then mounted onto glass slides.
  4. Deparaffinization: The paraffin wax is removed from the tissue sections using a series of xylene or other organic solvent washes.
  5. Antigen retrieval: To expose the prolactin protein for antibody binding, antigen retrieval is performed. This can be done by heating the tissue sections in a buffer solution or using enzymatic digestion.
  6. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking agent, such as bovine serum albumin or normal serum.
  7. Primary antibody incubation: The tissue sections are incubated with a primary antibody specific to prolactin. This antibody will bind to the prolactin protein if present in the tissue.
  8. Secondary antibody incubation: After washing off unbound primary antibody, a secondary antibody is applied. This secondary antibody is conjugated to a detection system, such as an enzyme or fluorophore.
  9. Visualization: The detection system on the secondary antibody reacts with a substrate, resulting in a visible signal. This signal can be visualized under a microscope or using other imaging techniques.
  10. Counterstaining: To enhance tissue contrast and provide additional information, a counterstain may be applied to the tissue sections. Hematoxylin is commonly used as a counterstain.
  11. Analysis: The tissue sections are examined under a microscope, and the presence and distribution of prolactin in the tissue sample are evaluated. This analysis can help diagnose prolactin-related conditions or study the role of prolactin in various tissues.

It is important to note that the specific protocol and reagents used for the immunohistochemistry prolactin test may vary depending on the laboratory and specific requirements of the study or diagnostic evaluation.

Test Name IMMUNOHISTOCHEMISTRY PROLACTIN Test
Components
Price 410.0 AED
Sample Condition Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Report Delivery Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days
Method Immunohistochemistry
Test type Cancer
Doctor Oncologist, Pathologist
Test Department:
Pre Test Information Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Test Details

Immunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples. In the case of the prolactin test, IHC can be used to identify the presence and distribution of prolactin in a tissue sample.

To perform an immunohistochemistry prolactin test, the following steps are typically followed:

1. Tissue fixation: The tissue sample is collected and fixed using a suitable fixative, such as formalin. This helps preserve the tissue structure and proteins.

2. Tissue processing: The fixed tissue is then processed by embedding it in paraffin wax, which allows for easier handling and sectioning.

3. Sectioning: Thin sections (usually around 4-6 micrometers) of the paraffin-embedded tissue are cut using a microtome. These sections are then mounted onto glass slides.

4. Deparaffinization: The paraffin wax is removed from the tissue sections using a series of xylene or other organic solvent washes.

5. Antigen retrieval: To expose the prolactin protein for antibody binding, antigen retrieval is performed. This can be done by heating the tissue sections in a buffer solution or using enzymatic digestion.

6. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking agent, such as bovine serum albumin or normal serum.

7. Primary antibody incubation: The tissue sections are incubated with a primary antibody specific to prolactin. This antibody will bind to the prolactin protein if present in the tissue.

8. Secondary antibody incubation: After washing off unbound primary antibody, a secondary antibody is applied. This secondary antibody is conjugated to a detection system, such as an enzyme or fluorophore.

9. Visualization: The detection system on the secondary antibody reacts with a substrate, resulting in a visible signal. This signal can be visualized under a microscope or using other imaging techniques.

10. Counterstaining: To enhance tissue contrast and provide additional information, a counterstain may be applied to the tissue sections. Hematoxylin is commonly used as a counterstain.

11. Analysis: The tissue sections are examined under a microscope, and the presence and distribution of prolactin in the tissue sample are evaluated. This analysis can help diagnose prolactin-related conditions or study the role of prolactin in various tissues.

It is important to note that the specific protocol and reagents used for the immunohistochemistry prolactin test may vary depending on the laboratory and specific requirements of the study or diagnostic evaluation.