Immunohistochemistry OLIG2 Test sale cost 470 AED
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Immunohistochemistry OLIG2 Test Cost

Original price was: 520 د.إ.Current price is: 470 د.إ.

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The Immunohistochemistry OLIG2 Test is a specialized diagnostic procedure performed to detect the presence of OLIG2, a protein that plays a crucial role in the development and function of the nervous system. OLIG2 is particularly significant in the study and diagnosis of various neurological conditions, including certain types of brain tumors. The test employs immunohistochemistry (IHC) techniques, which involve the use of antibodies to identify specific antigens in tissue samples, thereby allowing for precise localization of OLIG2 protein within the cells.

At DNA Labs UAE, this test is available for a cost of 470 AED. The lab utilizes state-of-the-art technology and adheres to stringent quality control measures to ensure accurate and reliable results. The Immunohistochemistry OLIG2 Test is instrumental for researchers and clinicians in the diagnosis, prognosis, and in some cases, the determination of therapeutic strategies for patients with neurological conditions linked to OLIG2 expression.

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IMMUNOHISTOCHEMISTRY OLIG2 Test

Components

Price: 470.0 AED

Sample Condition

Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Report Delivery

Sample: Daily by 6 pm

Report Block: 5 days

Tissue Biopsy: 5 days

Tissue large complex: 7 days

Method

Immunohistochemistry

Test type

Cancer

Doctor

Oncologist, Pathologist

Test Department

HISTOLOGY

Pre Test Information

Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Test Details

Olig2 (Oligodendrocyte transcription factor 2) is a transcription factor that plays a critical role in the development and maintenance of oligodendrocytes, which are the myelin-producing cells in the central nervous system.

Immunohistochemistry (IHC) is a technique used to visualize the presence and localization of specific proteins in tissue samples.

Steps involved in performing an Immunohistochemistry (IHC) Olig2 test:

  1. Tissue Preparation: Tissue samples are collected and fixed using formalin or other fixatives to preserve the cellular structure and protein integrity.
  2. Embedding: The fixed tissue is embedded in paraffin wax, which allows for thin sectioning of the tissue for subsequent staining.
  3. Sectioning: Thin sections (usually around 4-6 micrometers thick) are cut from the paraffin-embedded tissue blocks using a microtome.
  4. Deparaffinization and Rehydration: The paraffin is removed from the tissue sections using xylene or other clearing agents, followed by rehydration through a series of graded alcohol solutions.
  5. Antigen Retrieval: Heat-induced antigen retrieval is often performed to unmask the target antigen epitopes that may be masked by the fixation process. This can be achieved by boiling the tissue sections in a citrate buffer or using enzymatic digestion.
  6. Blocking: Non-specific binding sites are blocked using a blocking solution (e.g., serum or protein-based blocking reagents) to reduce background staining.
  7. Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific to Olig2. The primary antibody binds to the Olig2 protein in the tissue sections.
  8. Secondary Antibody Incubation: After washing off unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system (e.g., horseradish peroxidase or alkaline phosphatase). The secondary antibody binds to the primary antibody.
  9. Visualization: The detection system catalyzes a colorimetric reaction or produces a fluorescent signal, allowing the visualization of the Olig2 protein in the tissue sections.
  10. Counterstaining and Mounting: To enhance tissue contrast, counterstaining with dyes such as hematoxylin or eosin may be performed. The stained tissue sections are then mounted onto glass slides using a mounting medium.
  11. Microscopic Examination: The stained tissue sections are examined under a microscope to assess the presence and localization of Olig2 protein in the tissue.

The results of the Olig2 immunohistochemistry test can provide valuable information about the expression and distribution of Olig2 protein in the tissue sample, which can be useful for studying oligodendrocyte development, myelination, and diseases involving oligodendrocyte dysfunction or loss.

Test Name IMMUNOHISTOCHEMISTRY OLIG2 Test
Components
Price 470.0 AED
Sample Condition Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Report Delivery Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days
Method Immunohistochemistry
Test type Cancer
Doctor Oncologist, Pathologist
Test Department: HISTOLOGY
Pre Test Information Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Test Details

Olig2 (Oligodendrocyte transcription factor 2) is a transcription factor that plays a critical role in the development and maintenance of oligodendrocytes, which are the myelin-producing cells in the central nervous system. Immunohistochemistry (IHC) is a technique used to visualize the presence and localization of specific proteins in tissue samples.

To perform an immunohistochemistry (IHC) Olig2 test, the following steps are typically involved:

1. Tissue Preparation: Tissue samples are collected and fixed using formalin or other fixatives to preserve the cellular structure and protein integrity.

2. Embedding: The fixed tissue is embedded in paraffin wax, which allows for thin sectioning of the tissue for subsequent staining.

3. Sectioning: Thin sections (usually around 4-6 micrometers thick) are cut from the paraffin-embedded tissue blocks using a microtome.

4. Deparaffinization and Rehydration: The paraffin is removed from the tissue sections using xylene or other clearing agents, followed by rehydration through a series of graded alcohol solutions.

5. Antigen Retrieval: Heat-induced antigen retrieval is often performed to unmask the target antigen epitopes that may be masked by the fixation process. This can be achieved by boiling the tissue sections in a citrate buffer or using enzymatic digestion.

6. Blocking: Non-specific binding sites are blocked using a blocking solution (e.g., serum or protein-based blocking reagents) to reduce background staining.

7. Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific to Olig2. The primary antibody binds to the Olig2 protein in the tissue sections.

8. Secondary Antibody Incubation: After washing off unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system (e.g., horseradish peroxidase or alkaline phosphatase). The secondary antibody binds to the primary antibody.

9. Visualization: The detection system catalyzes a colorimetric reaction or produces a fluorescent signal, allowing the visualization of the Olig2 protein in the tissue sections.

10. Counterstaining and Mounting: To enhance tissue contrast, counterstaining with dyes such as hematoxylin or eosin may be performed. The stained tissue sections are then mounted onto glass slides using a mounting medium.

11. Microscopic Examination: The stained tissue sections are examined under a microscope to assess the presence and localization of Olig2 protein in the tissue.

The results of the Olig2 immunohistochemistry test can provide valuable information about the expression and distribution of Olig2 protein in the tissue sample, which can be useful for studying oligodendrocyte development, myelination, and diseases involving oligodendrocyte dysfunction or loss.

SKU: TEST-6392