IMMUNOHISTOCHEMISTRY NEURON SPECIFIC ENOLASE NSE Test
Test Name: IMMUNOHISTOCHEMISTRY NEURON SPECIFIC ENOLASE NSE Test
Components: Neuron Specific Enolase (NSE) Test
Price: 410.0 AED
Sample Condition: Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Report Delivery: Sample Daily by 6 pm; Report Block: 5 days, Tissue Biopsy: 5 days, Tissue large complex: 7 days
Method: Immunohistochemistry
Test type: Cancer
Doctor: Oncologist, Pathologist
Test Department: DNA Labs UAE
Pre Test Information: Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Test Details:
Immunohistochemistry is a technique used to detect specific proteins in tissue sections. Neuron-specific enolase (NSE) is a protein that is primarily found in neurons and neuroendocrine cells. The NSE test in immunohistochemistry involves using specific antibodies that bind to NSE protein in tissue sections.
The NSE test is commonly used in the diagnosis and evaluation of various neurological disorders, including brain tumors, neuroendocrine tumors, and neurodegenerative diseases. It helps in identifying and characterizing different types of neurons and neuroendocrine cells in tissue samples.
The NSE test procedure involves the following steps:
- Tissue fixation: The tissue sample is first fixed using a suitable fixative, such as formalin, to preserve the cellular structure.
- Tissue processing: The fixed tissue is then processed to embed it in paraffin wax, which allows thin sections to be cut for further analysis.
- Sectioning: Thin sections (usually around 4-5 micrometers) are cut from the paraffin-embedded tissue block using a microtome.
- Deparaffinization: The paraffin wax is removed from the tissue sections by treating them with xylene or other suitable solvents.
- Antigen retrieval: In some cases, antigen retrieval may be required to unmask the NSE protein epitopes and improve antibody binding. This can be done by treating the tissue sections with heat or enzymes.
- Blocking: The tissue sections are then treated with a blocking solution, such as serum or protein blocking agents, to prevent non-specific antibody binding.
- Primary antibody incubation: The tissue sections are incubated with a primary antibody specific to NSE. This antibody binds to the NSE protein present in neurons and neuroendocrine cells.
- Secondary antibody incubation: After washing off any unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system, such as a fluorescent dye or an enzyme.
- Visualization: The detection system attached to the secondary antibody produces a visible signal that indicates the presence of NSE protein in the tissue sections. This can be visualized using a fluorescence microscope or by converting the signal into a color reaction using appropriate substrates.
- Interpretation: The stained tissue sections are examined under a microscope by a pathologist who interprets the results based on the presence or absence of NSE staining in neurons and neuroendocrine cells.
The NSE test is a valuable tool in neuropathology and can provide important diagnostic and prognostic information for various neurological conditions. However, it is important to note that the NSE test should be interpreted in conjunction with other clinical and histopathological findings to make an accurate diagnosis.
| Test Name | IMMUNOHISTOCHEMISTRY NEURON SPECIFIC ENOLASE NSE Test |
|---|---|
| Components | |
| Price | 410.0 AED |
| Sample Condition | Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history. |
| Report Delivery | Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days |
| Method | Immunohistochemistry |
| Test type | Cancer |
| Doctor | Oncologist, Pathologist |
| Test Department: | |
| Pre Test Information | Provide a copy of the Histopathology report, Site of biopsy and Clinical history. |
| Test Details | Immunohistochemistry is a technique used to detect specific proteins in tissue sections. Neuron-specific enolase (NSE) is a protein that is primarily found in neurons and neuroendocrine cells. The NSE test in immunohistochemistry involves using specific antibodies that bind to NSE protein in tissue sections. The NSE test is commonly used in the diagnosis and evaluation of various neurological disorders, including brain tumors, neuroendocrine tumors, and neurodegenerative diseases. It helps in identifying and characterizing different types of neurons and neuroendocrine cells in tissue samples. The NSE test procedure involves the following steps: 1. Tissue fixation: The tissue sample is first fixed using a suitable fixative, such as formalin, to preserve the cellular structure. 2. Tissue processing: The fixed tissue is then processed to embed it in paraffin wax, which allows thin sections to be cut for further analysis. 3. Sectioning: Thin sections (usually around 4-5 micrometers) are cut from the paraffin-embedded tissue block using a microtome. 4. Deparaffinization: The paraffin wax is removed from the tissue sections by treating them with xylene or other suitable solvents. 5. Antigen retrieval: In some cases, antigen retrieval may be required to unmask the NSE protein epitopes and improve antibody binding. This can be done by treating the tissue sections with heat or enzymes. 6. Blocking: The tissue sections are then treated with a blocking solution, such as serum or protein blocking agents, to prevent non-specific antibody binding. 7. Primary antibody incubation: The tissue sections are incubated with a primary antibody specific to NSE. This antibody binds to the NSE protein present in neurons and neuroendocrine cells. 8. Secondary antibody incubation: After washing off any unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system, such as a fluorescent dye or an enzyme. 9. Visualization: The detection system attached to the secondary antibody produces a visible signal that indicates the presence of NSE protein in the tissue sections. This can be visualized using a fluorescence microscope or by converting the signal into a color reaction using appropriate substrates. 10. Interpretation: The stained tissue sections are examined under a microscope by a pathologist who interprets the results based on the presence or absence of NSE staining in neurons and neuroendocrine cells. The NSE test is a valuable tool in neuropathology and can provide important diagnostic and prognostic information for various neurological conditions. However, it is important to note that the NSE test should be interpreted in conjunction with other clinical and histopathological findings to make an accurate diagnosis. |
