Immunohistochemistry LIN-28 Test sale cost 510 AED
Infertility Comprehensive Panel Females Test sale cost 540 AED Infertility Comprehensive Panel Females Test Cost 600 د.إ Original price was: 600 د.إ.540 د.إCurrent price is: 540 د.إ.
Lp-PLA2 Lipoprotein Associated Phospholipase A2 Activity Test sale cost 510 AED Lp-PLA2 Lipoprotein Associated Phospholipase A2 Activity Test Cost 570 د.إ Original price was: 570 د.إ.510 د.إCurrent price is: 510 د.إ.
Sale!

Immunohistochemistry LIN-28 Test Cost

Original price was: 570 د.إ.Current price is: 510 د.إ.

-11%

The Immunohistochemistry LIN-28 Test is a specialized diagnostic procedure available at DNA Labs UAE, priced at 510 AED. This test employs immunohistochemistry (IHC) techniques to detect the presence and localization of the LIN-28 protein within tissue samples. LIN-28 is a biomarker that plays a critical role in stem cell renewal and development, and its expression can be indicative of various types of cancer, including germ cell tumors and certain types of sarcomas. By identifying the presence of LIN-28, healthcare professionals can gain valuable insights into the nature of the disease, aiding in diagnosis, prognostication, and the tailoring of treatment strategies. The test involves the application of specific antibodies to tissue sections, which bind to the LIN-28 protein if present, and can be visualized under a microscope. Conducted in the state-of-the-art facilities of DNA Labs UAE, this test represents a crucial step in the management and understanding of certain cancers and developmental disorders.

Home  Sample collection service available

  • 100% accuaret Test Results
  • Ranked as Most trusted Genetic DNA Lab
  • This test is not intended for medical diagnosis or treatment
Guaranteed Safe Checkout

IMMUNOHISTOCHEMISTRY LIN-28 Test

Cost: AED 510.0

Symptoms, Diagnosis, and Test Details

Are you experiencing symptoms that require further investigation? The IMMUNOHISTOCHEMISTRY LIN-28 Test offered by DNA Labs UAE might provide the answers you’re looking for. This test is specifically designed to detect specific proteins in tissue samples, helping doctors diagnose cancer and understand its progression.

Test Components and Price

The IMMUNOHISTOCHEMISTRY LIN-28 Test is priced at AED 510.0. It involves analyzing tumor tissue samples using immunohistochemistry techniques to identify the LIN-28 protein.

Sample Condition and Submission

To ensure accurate results, it is crucial to submit tumor tissue in either 10% Formal-saline or a Formalin-fixed paraffin-embedded block. The samples should be shipped at room temperature. Along with the samples, provide a copy of the Histopathology report, site of biopsy, and clinical history for a comprehensive analysis.

Report Delivery

The report for the IMMUNOHISTOCHEMISTRY LIN-28 Test will be delivered daily by 6 pm. The turnaround time for different sample types is as follows: Report Block: 5 days, Tissue Biopsy: 5 days, and Tissue large complex: 7 days.

Method and Test Type

The IMMUNOHISTOCHEMISTRY LIN-28 Test is performed using immunohistochemistry, a technique that detects specific proteins in tissue samples. This test specifically focuses on LIN-28, a protein marker commonly used to study its expression in various tissues. The test falls under the category of cancer diagnostics and is typically requested by oncologists.

Pre Test Information

Prior to the test, it is essential to provide a copy of the Histopathology report, site of biopsy, and clinical history. These details assist the laboratory in analyzing the samples accurately.

Test Procedure

The IMMUNOHISTOCHEMISTRY LIN-28 Test follows a specific procedure to ensure accurate results:

  1. Tissue preparation: Tissue samples obtained from biopsies or surgical resections are fixed in formalin and embedded in paraffin wax. Thin sections of the tissue are then cut using a microtome.
  2. Deparaffinization and rehydration: The paraffin-embedded tissue sections are deparaffinized using appropriate solvents like xylene. They are then rehydrated using decreasing concentrations of alcohol.
  3. Antigen retrieval: The tissue sections are treated with an antigen retrieval solution to unmask the target antigen and improve antibody binding.
  4. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking buffer containing a protein such as bovine serum albumin (BSA) or normal serum.
  5. Primary antibody incubation: The tissue sections are incubated with a primary antibody specific to LIN-28, allowing it to recognize and bind to the target protein.
  6. Secondary antibody incubation: After washing off unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system, amplifying the signal for visualization.
  7. Visualization: The tissue sections are visualized either under a fluorescence microscope if a fluorescent dye is used or by adding a chromogenic substrate for an enzyme-based detection system.
  8. Counterstaining: In some cases, counterstaining with a dye like hematoxylin is performed to visualize cellular structures and aid in interpretation.
  9. Mounting: The stained tissue sections are mounted onto glass slides using a resin-based mounting medium to preserve the staining and protect the tissue.
  10. Analysis: The stained tissue sections are examined under a microscope to analyze and interpret the expression and localization of the LIN-28 protein.

The IMMUNOHISTOCHEMISTRY LIN-28 Test provides valuable information about the expression pattern and localization of LIN-28 protein in different tissues. This information helps researchers and clinicians understand its role in various biological processes and diseases.

Conclusion

If you suspect cancer or require further investigation into specific proteins, the IMMUNOHISTOCHEMISTRY LIN-28 Test offered by DNA Labs UAE is a reliable option. By analyzing tissue samples, this test provides insights into the expression and localization of LIN-28 protein, aiding in accurate diagnosis and treatment decisions.

Test Name IMMUNOHISTOCHEMISTRY LIN-28 Test
Components
Price 510.0 AED
Sample Condition Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Report Delivery Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days
Method Immunohistochemistry
Test type Cancer
Doctor Oncologist
Test Department: HISTOLOGY
Pre Test Information Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Test Details

Immunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples. The LIN-28 protein is a marker that is commonly used in IHC to study its expression in various tissues.

To perform an immunohistochemistry LIN-28 test, the following steps are typically followed:

1. Tissue preparation: Tissue samples, usually obtained from biopsies or surgical resections, are fixed in formalin and embedded in paraffin wax. The tissue is then cut into thin sections (usually 4-6 micrometers thick) using a microtome.

2. Deparaffinization and rehydration: The paraffin-embedded tissue sections are deparaffinized by incubating them in xylene or other appropriate solvents. The sections are then rehydrated by passing them through a series of decreasing concentrations of alcohol.

3. Antigen retrieval: The tissue sections are treated with an antigen retrieval solution, such as heat-induced epitope retrieval or enzyme-induced epitope retrieval, to unmask the target antigen and improve antibody binding.

4. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking buffer, typically containing a protein such as bovine serum albumin (BSA) or normal serum.

5. Primary antibody incubation: The tissue sections are incubated with a primary antibody specific to LIN-28. The primary antibody recognizes and binds to the target protein in the tissue sections.

6. Secondary antibody incubation: After washing off unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system, such as a fluorescent dye or an enzyme. The secondary antibody binds to the primary antibody, amplifying the signal and allowing visualization of the target protein.

7. Visualization: If an enzyme-based detection system is used, a chromogenic substrate is added, which reacts with the enzyme to produce a visible color change. If a fluorescent dye is used, the tissue sections are directly visualized under a fluorescence microscope.

8. Counterstaining: In some cases, counterstaining with a dye, such as hematoxylin, is performed to visualize the cellular structure and aid in interpretation.

9. Mounting: The stained tissue sections are mounted onto glass slides using a mounting medium, such as a resin, to preserve the staining and protect the tissue.

10. Analysis: The stained tissue sections are then examined under a microscope, and the expression and localization of LIN-28 protein in the tissue are analyzed and interpreted.

The immunohistochemistry LIN-28 test can provide valuable information about the expression pattern and localization of LIN-28 protein in different tissues, helping researchers and clinicians understand its role in various biological processes and diseases.

SKU: TEST-6254