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Immunohistochemistry Glutamine Synthetase Test Cost

Original price was: 570 د.إ.Current price is: 510 د.إ.

-11%

The Immunohistochemistry Glutamine Synthetase Test is a specialized diagnostic procedure employed to detect the presence and distribution of glutamine synthetase, an enzyme crucial in the metabolism of ammonia and the biosynthesis of glutamine, within tissue samples. This test is particularly useful in medical research and clinical diagnostics for conditions related to liver function, as glutamine synthetase plays a key role in the liver’s ammonia detoxification pathway. It is also applied in the investigation of certain brain disorders and tumors, given the enzyme’s significant expression in these areas.

Performed at DNA Labs UAE, a leading facility known for its advanced diagnostic technologies and expert medical staff, the test involves the application of immunohistochemistry techniques. These techniques utilize antibodies specific to glutamine synthetase to bind to the enzyme in tissue sections, which are then visualized under a microscope through the use of dyes or fluorescent markers. This allows for the precise localization and quantification of glutamine synthetase in the tissues examined.

The cost of the Immunohistochemistry Glutamine Synthetase Test at DNA Labs UAE is 510 AED. This price reflects the intricate nature of the test, including the sophisticated materials and expertise required to accurately perform the analysis and interpret the results. Patients and healthcare providers considering this test can expect a high level of diagnostic accuracy and valuable insights into the conditions being investigated, supported by the reputable services of DNA Labs UAE.

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IMMUNOHISTOCHEMISTRY GLUTAMINE SYNTHETASE Test

Test Cost: AED 510.0

Symptoms, Diagnosis, and Referring Details:

Test Name: IMMUNOHISTOCHEMISTRY GLUTAMINE SYNTHETASE Test

Components: Price 510.0 AED

Sample Condition: Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Report Delivery: Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex: 7 days

Method: Immunohistochemistry

Test Type: Cancer

Doctor: Oncologist

Test Department: HISTOLOGY

Pre Test Information: Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Test Details:

Immunohistochemistry (IHC) is a technique used to visualize specific proteins or antigens in tissue sections using antibodies that specifically bind to the target protein. Glutamine synthetase (GS) is an enzyme involved in the synthesis of the amino acid glutamine.

To perform an immunohistochemistry test for glutamine synthetase, the following steps can be followed:

  1. Tissue Preparation: Tissue samples are collected and fixed in a suitable fixative, such as formalin. The fixed tissues are then embedded in paraffin wax or frozen for sectioning.
  2. Sectioning: The fixed tissues are sectioned into thin slices using a microtome. The sections are then mounted onto glass slides.
  3. Deparaffinization (if applicable): If paraffin-embedded sections are used, the slides are deparaffinized by immersing them in xylene or other suitable solvents. This step is skipped for frozen sections.
  4. Antigen Retrieval: In order to expose the target antigen, heat-induced epitope retrieval or enzymatic digestion methods can be used. This step is essential for formalin-fixed paraffin-embedded (FFPE) tissues.
  5. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking buffer, typically containing serum or protein-based solutions.
  6. Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific for glutamine synthetase. The primary antibody binds to the target protein in the tissue sections.
  7. Secondary Antibody Incubation: After washing off unbound primary antibody, a secondary antibody conjugated to a detection molecule, such as a fluorescent dye or an enzyme, is applied. The secondary antibody binds to the primary antibody.
  8. Visualization: If an enzyme-conjugated secondary antibody is used, a chromogenic substrate is added, which produces a colored precipitate where the target protein is present. If a fluorescent dye-conjugated secondary antibody is used, the sections are directly visualized under a fluorescence microscope.
  9. Counterstaining (optional): To enhance the contrast and visualization of the tissue sections, a counterstain, such as hematoxylin, may be applied.
  10. Mounting: The slides are coverslipped using a mounting medium to preserve the stained tissue sections.

After the immunohistochemistry test, the tissue sections can be examined under a microscope to observe the presence and distribution of glutamine synthetase in the tissue. The staining pattern and intensity can provide information about the expression and localization of the protein in the tissue sample.

Test Name IMMUNOHISTOCHEMISTRY GLUTAMINE SYNTHETASE Test
Components
Price 510.0 AED
Sample Condition Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Report Delivery Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days
Method Immunohistochemistry
Test type Cancer
Doctor Oncologist
Test Department: HISTOLOGY
Pre Test Information Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Test Details

Immunohistochemistry (IHC) is a technique used to visualize specific proteins or antigens in tissue sections using antibodies that specifically bind to the target protein. Glutamine synthetase (GS) is an enzyme involved in the synthesis of the amino acid glutamine.

To perform an immunohistochemistry test for glutamine synthetase, the following steps can be followed:

1. Tissue Preparation: Tissue samples are collected and fixed in a suitable fixative, such as formalin. The fixed tissues are then embedded in paraffin wax or frozen for sectioning.

2. Sectioning: The fixed tissues are sectioned into thin slices using a microtome. The sections are then mounted onto glass slides.

3. Deparaffinization (if applicable): If paraffin-embedded sections are used, the slides are deparaffinized by immersing them in xylene or other suitable solvents. This step is skipped for frozen sections.

4. Antigen Retrieval: In order to expose the target antigen, heat-induced epitope retrieval or enzymatic digestion methods can be used. This step is essential for formalin-fixed paraffin-embedded (FFPE) tissues.

5. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking buffer, typically containing serum or protein-based solutions.

6. Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific for glutamine synthetase. The primary antibody binds to the target protein in the tissue sections.

7. Secondary Antibody Incubation: After washing off unbound primary antibody, a secondary antibody conjugated to a detection molecule, such as a fluorescent dye or an enzyme, is applied. The secondary antibody binds to the primary antibody.

8. Visualization: If an enzyme-conjugated secondary antibody is used, a chromogenic substrate is added, which produces a colored precipitate where the target protein is present. If a fluorescent dye-conjugated secondary antibody is used, the sections are directly visualized under a fluorescence microscope.

9. Counterstaining (optional): To enhance the contrast and visualization of the tissue sections, a counterstain, such as hematoxylin, may be applied.

10. Mounting: The slides are coverslipped using a mounting medium to preserve the stained tissue sections.

After the immunohistochemistry test, the tissue sections can be examined under a microscope to observe the presence and distribution of glutamine synthetase in the tissue. The staining pattern and intensity can provide information about the expression and localization of the protein in the tissue sample.

SKU: TEST-6260