Immunohistochemistry CD5 Test sale cost 410 AED
Immunohistochemistry CD138 Test sale cost 410 AED Immunohistochemistry CD138 Test Cost 460 د.إ Original price was: 460 د.إ.410 د.إCurrent price is: 410 د.إ.
Immunohistochemistry CD34 Test sale cost 410 AED Immunohistochemistry CD34 Test Cost 460 د.إ Original price was: 460 د.إ.410 د.إCurrent price is: 410 د.إ.
Sale!

Immunohistochemistry CD5 Test Cost

Original price was: 460 د.إ.Current price is: 410 د.إ.

-11%

Immunohistochemistry (IHC) CD5 Test is a specialized diagnostic procedure used to detect the presence of CD5 protein on the surface of cells, primarily in tissue samples. This test is particularly important in the diagnosis and classification of various hematologic malignancies, including certain types of lymphomas and leukemias, where CD5 expression can be a critical factor in determining the specific type of cancer and guiding treatment decisions. The test involves using antibodies that specifically bind to the CD5 antigen, followed by visualization under a microscope, allowing pathologists to assess the presence and distribution of CD5-expressing cells.

At DNA Labs UAE, the Immunohistochemistry CD5 Test is offered for a cost of 410 AED. The lab utilizes state-of-the-art technology and follows stringent protocols to ensure accurate and reliable results. Patients or healthcare providers looking to avail of this test at DNA Labs UAE can expect professional service and timely delivery of results, aiding in the efficient diagnosis and management of conditions associated with CD5 expression.

Home  Sample collection service available

  • 100% accuaret Test Results
  • Ranked as Most trusted Genetic DNA Lab
  • This test is not intended for medical diagnosis or treatment
Guaranteed Safe Checkout

IMMUNOHISTOCHEMISTRY CD5 Test

Test Name: IMMUNOHISTOCHEMISTRY CD5 Test

Components: CD5 protein marker

Price: 410.0 AED

Sample Condition: Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report. Indicate site of biopsy and provide Clinical history.

Report Delivery: Sample Daily by 6 pm; Report Block: 5 days, Tissue Biopsy: 5 days, Tissue large complex: 7 days

Method: Immunohistochemistry

Test Type: Cancer

Doctor: Oncologist, Pathologist

Test Department: DNA Labs UAE

Pre Test Information: Provide a copy of the Histopathology report. Indicate site of biopsy and provide Clinical history.

Test Details

CD5 is a protein marker that is commonly used in immunohistochemistry to identify T cells and B-1 cells. CD5 is expressed on the surface of mature T cells, as well as a subset of B cells known as B-1 cells.

To perform an immunohistochemistry CD5 test, the following steps are typically followed:

  1. Tissue Preparation: The tissue sample, usually obtained from a biopsy or surgical resection, is fixed in formalin and embedded in paraffin wax. The tissue is then cut into thin sections (4-5 micrometers) using a microtome.
  2. Deparaffinization and Rehydration: The paraffin wax is removed from the tissue sections by immersing them in xylene or a xylene substitute. The sections are then rehydrated by passing them through a series of graded alcohols (e.g., 100%, 95%, 70% ethanol).
  3. Antigen Retrieval: To enhance the accessibility of the CD5 antigen for antibody binding, antigen retrieval is performed. This is typically achieved by heat-induced epitope retrieval (HIER) using a citrate buffer or EDTA buffer. The tissue sections are heated in the retrieval buffer using a microwave or pressure cooker.
  4. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking solution, such as normal serum or bovine serum albumin (BSA). This helps to reduce background staining.
  5. Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific to CD5. The primary antibody is typically a monoclonal antibody that recognizes the CD5 antigen on T cells and B-1 cells. The sections are incubated overnight at 4C or for a shorter period at room temperature.
  6. Secondary Antibody Incubation: After washing away unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system. This secondary antibody recognizes the primary antibody and amplifies the signal. The detection system can be visualized using various methods, such as enzyme-linked immunosorbent assay (ELISA), immunofluorescence, or chromogenic detection.
  7. Visualization: The CD5 antigen is visualized by adding a chromogenic substrate or a fluorescent dye, depending on the detection system used. For chromogenic detection, a substrate such as diaminobenzidine (DAB) is added, which produces a brown color at the site of CD5 expression. For immunofluorescence, a fluorophore-conjugated secondary antibody is used, which emits fluorescence when excited by a specific wavelength of light.
  8. Counterstaining and Mounting: To enhance the contrast and visualization of the tissue sections, a counterstain, such as hematoxylin, may be applied. The stained tissue sections are then dehydrated, cleared, and mounted on glass slides using a mounting medium.
  9. Analysis: The stained tissue sections are examined under a microscope to evaluate the presence and distribution of CD5-positive cells. The intensity and pattern of staining can provide valuable information about the cellular composition and localization of CD5-expressing cells in the tissue sample.

Overall, the immunohistochemistry CD5 test is a valuable tool for studying the presence and distribution of CD5-positive cells, particularly T cells and B-1 cells, in tissue samples. It is commonly used in research and diagnostic settings to aid in the diagnosis and classification of various diseases, such as lymphomas and autoimmune disorders.

Test Name IMMUNOHISTOCHEMISTRY CD5 Test
Components
Price 410.0 AED
Sample Condition Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report. Indicate site of biopsy and provide Clinical history.
Report Delivery Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days
Method Immunohistochemistry
Test type Cancer
Doctor Oncologist, Pathologist
Test Department:
Pre Test Information Provide a copy of the Histopathology report. Indicate site of biopsy and provide Clinical history.
Test Details

CD5 is a protein marker that is commonly used in immunohistochemistry to identify T cells and B-1 cells. CD5 is expressed on the surface of mature T cells, as well as a subset of B cells known as B-1 cells.

To perform an immunohistochemistry CD5 test, the following steps are typically followed:

1. Tissue Preparation: The tissue sample, usually obtained from a biopsy or surgical resection, is fixed in formalin and embedded in paraffin wax. The tissue is then cut into thin sections (4-5 micrometers) using a microtome.

2. Deparaffinization and Rehydration: The paraffin wax is removed from the tissue sections by immersing them in xylene or a xylene substitute. The sections are then rehydrated by passing them through a series of graded alcohols (e.g., 100%, 95%, 70% ethanol).

3. Antigen Retrieval: To enhance the accessibility of the CD5 antigen for antibody binding, antigen retrieval is performed. This is typically achieved by heat-induced epitope retrieval (HIER) using a citrate buffer or EDTA buffer. The tissue sections are heated in the retrieval buffer using a microwave or pressure cooker.

4. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking solution, such as normal serum or bovine serum albumin (BSA). This helps to reduce background staining.

5. Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific to CD5. The primary antibody is typically a monoclonal antibody that recognizes the CD5 antigen on T cells and B-1 cells. The sections are incubated overnight at 4C or for a shorter period at room temperature.

6. Secondary Antibody Incubation: After washing away unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system. This secondary antibody recognizes the primary antibody and amplifies the signal. The detection system can be visualized using various methods, such as enzyme-linked immunosorbent assay (ELISA), immunofluorescence, or chromogenic detection.

7. Visualization: The CD5 antigen is visualized by adding a chromogenic substrate or a fluorescent dye, depending on the detection system used. For chromogenic detection, a substrate such as diaminobenzidine (DAB) is added, which produces a brown color at the site of CD5 expression. For immunofluorescence, a fluorophore-conjugated secondary antibody is used, which emits fluorescence when excited by a specific wavelength of light.

8. Counterstaining and Mounting: To enhance the contrast and visualization of the tissue sections, a counterstain, such as hematoxylin, may be applied. The stained tissue sections are then dehydrated, cleared, and mounted on glass slides using a mounting medium.

9. Analysis: The stained tissue sections are examined under a microscope to evaluate the presence and distribution of CD5-positive cells. The intensity and pattern of staining can provide valuable information about the cellular composition and localization of CD5-expressing cells in the tissue sample.

Overall, the immunohistochemistry CD5 test is a valuable tool for studying the presence and distribution of CD5-positive cells, particularly T cells and B-1 cells, in tissue samples. It is commonly used in research and diagnostic settings to aid in the diagnosis and classification of various diseases, such as lymphomas and autoimmune disorders.

SKU: TEST-6468 Category:

Related products