IMMUNOHISTOCHEMISTRY CD35 Test
Welcome to DNA Labs UAE, where we offer the IMMUNOHISTOCHEMISTRY CD35 Test. This test is used for diagnosing cancer and is performed using immunohistochemistry (IHC) to detect the presence and localization of the CD35 protein in tissue samples.
Test Details
CD35, also known as complement receptor 1 (CR1), is a cell surface protein involved in the regulation of the complement system. The IMMUNOHISTOCHEMISTRY CD35 Test helps determine the expression and distribution of CD35 in the tissue, providing valuable information for diagnosis.
Components and Price
The IMMUNOHISTOCHEMISTRY CD35 Test is priced at 410.0 AED.
Sample Condition and Submission
To perform the test, please submit tumor tissue in 10% Formal-saline or a Formalin-fixed paraffin-embedded block. The sample should be shipped at room temperature. Additionally, provide a copy of the Histopathology report, the site of biopsy, and the clinical history.
Report Delivery
The sample is processed daily, and the report is delivered by 6 pm. The turnaround time for the report is 5 days for blocks and tissue biopsies, and 7 days for tissue large complex.
Test Method
The IMMUNOHISTOCHEMISTRY CD35 Test utilizes immunohistochemistry as the method to detect the CD35 protein. The following steps are involved:
- Tissue Preparation: Tissue samples are fixed in formalin and embedded in paraffin wax or can be frozen.
- Deparaffinization and Rehydration: If paraffin-embedded tissue sections are used, the paraffin wax is removed, and the slides are rehydrated.
- Antigen Retrieval: Heat-induced antigen retrieval is performed to expose the CD35 antigen for antibody binding.
- Blocking: Non-specific binding sites on the tissue sections are blocked to reduce background staining.
- Primary Antibody Incubation: Tissue sections are incubated with a primary antibody specific to CD35.
- Secondary Antibody Incubation: Tissue sections are incubated with a secondary antibody conjugated to a detection system.
- Visualization: The detection system can be enzymatic or fluorescent.
- Counterstaining and Mounting: A counterstain, such as hematoxylin, may be applied to enhance tissue structure visualization. The tissue sections are then mounted on glass slides.
- Microscopic Examination: The stained tissue sections are examined under a microscope to visualize the presence and localization of CD35.
It is important to note that the specific protocol and reagents used for the IMMUNOHISTOCHEMISTRY CD35 Test may vary depending on the laboratory and manufacturer’s instructions for the primary and secondary antibodies.
Test Department and Doctors
The IMMUNOHISTOCHEMISTRY CD35 Test is conducted in our Test Department. Our team of experienced doctors, including Oncologists and Pathologists, oversee the testing process to ensure accurate results.
Pre Test Information
Prior to the test, please provide a copy of the Histopathology report, the site of biopsy, and the clinical history. This information is essential for a comprehensive evaluation.
At DNA Labs UAE, we are committed to providing reliable and accurate testing services. Contact us today to schedule your IMMUNOHISTOCHEMISTRY CD35 Test.
| Test Name | IMMUNOHISTOCHEMISTRY CD35 Test |
|---|---|
| Components | |
| Price | 410.0 AED |
| Sample Condition | Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history. |
| Report Delivery | Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days |
| Method | Immunohistochemistry |
| Test type | Cancer |
| Doctor | Oncologist, Pathologist |
| Test Department: | |
| Pre Test Information | Provide a copy of the Histopathology report, Site of biopsy and Clinical history. |
| Test Details |
CD35, also known as complement receptor 1 (CR1), is a cell surface protein involved in the regulation of the complement system. Immunohistochemistry (IHC) is a technique used to detect the presence and localization of specific proteins in tissue samples. To perform an immunohistochemistry CD35 test, the following steps are typically involved: 1. Tissue Preparation: Tissue samples, usually obtained from biopsy or surgical resection, are fixed in formalin and embedded in paraffin wax. Alternatively, frozen tissue sections can also be used. 2. Deparaffinization and Rehydration: If paraffin-embedded tissue sections are used, the paraffin wax is removed by immersing the slides in xylene or a xylene substitute, followed by rehydration through a series of graded alcohols. 3. Antigen Retrieval: Heat-induced antigen retrieval is often required to expose the CD35 antigen for antibody binding. This can be achieved by boiling the tissue sections in a buffer solution or using enzymatic digestion methods. 4. Blocking: Non-specific binding sites on the tissue sections are blocked using a blocking solution, typically containing serum or protein blocking agents. This helps reduce background staining. 5. Primary Antibody Incubation: The tissue sections are incubated with a primary antibody specific to CD35. The primary antibody can be monoclonal or polyclonal, depending on the specific test requirements. Incubation time and temperature may vary depending on the antibody used. 6. Secondary Antibody Incubation: After washing off unbound primary antibody, the tissue sections are incubated with a secondary antibody conjugated to a detection system. This secondary antibody recognizes and binds to the primary antibody, amplifying the signal for detection. 7. Visualization: The detection system can be enzymatic or fluorescent, depending on the intended visualization method. Enzymatic detection systems often involve the use of a chromogenic substrate that produces a visible color change when acted upon by the enzyme conjugated to the secondary antibody. Fluorescent detection systems utilize fluorophore-conjugated secondary antibodies that emit fluorescence when excited by a specific wavelength of light. 8. Counterstaining and Mounting: To enhance tissue structure visualization, a counterstain, such as hematoxylin, may be applied. Finally, the tissue sections are mounted on glass slides using a mounting medium. 9. Microscopic Examination: The stained tissue sections are examined under a microscope to visualize the presence and localization of CD35. The staining pattern and intensity can provide information about the expression and distribution of CD35 in the tissue. It is important to note that the specific protocol and reagents used for an immunohistochemistry CD35 test may vary depending on the laboratory and the manufacturer’s instructions for the primary and secondary antibodies. |
