HEPATITIS D VIRUS DETECTION PCR Test
Test Name: HEPATITIS D VIRUS DETECTION PCR Test
Components: Price: 1000.0 AED
Sample Condition: 4 mL (2 mL min.) plasma from 1 Lavender Top (EDTA) tube. Separate plasma aseptically within 2 hours of collection. Ship refrigerated or frozen. 12 hours fasting is recommended.
Report Delivery: Sample Daily by 11 am; Report 13 days
Method: PCR
Test type: Disorders of Liver
Doctor: Gastroenterologist
Test Department: MOLECULAR DIAGNOSTICS
Pre Test Information: 12 hours fasting is recommended.
Test Details:
The PCR test is a highly sensitive and specific method used to detect the presence of the hepatitis D virus (HDV) in a person’s blood. PCR stands for polymerase chain reaction, which is a laboratory technique that amplifies specific segments of DNA or RNA. In the case of HDV detection, the PCR test targets and amplifies the viral RNA present in the blood sample. This amplification allows for the detection of even small amounts of the virus, making the PCR test a reliable method for diagnosing hepatitis D.
The PCR test for HDV detection involves several steps. First, the viral RNA is extracted from the blood sample. Then, specific primers and probes that are complementary to the viral RNA are added to the sample. These primers and probes bind to the viral RNA if it is present. Next, an enzyme called reverse transcriptase is used to convert the viral RNA into complementary DNA (cDNA). This cDNA is then amplified using a DNA polymerase enzyme, creating multiple copies of the viral DNA. The amplification process is carried out through a series of temperature cycles, which allow the DNA polymerase enzyme to synthesize new DNA strands. Each cycle doubles the amount of DNA, resulting in a significant increase in the amount of viral DNA if the virus is present.
Finally, the amplified DNA is analyzed using various methods, such as gel electrophoresis or fluorescent probes, to determine the presence or absence of the hepatitis D virus.
Overall, the PCR test for hepatitis D virus detection is a sensitive and specific method that allows for early diagnosis and monitoring of HDV infection. It is a valuable tool in the management of hepatitis D and helps in determining appropriate treatment options.
| Test Name | HEPATITIS D VIRUS DETECTION PCR Test |
|---|---|
| Components | |
| Price | 1000.0 AED |
| Sample Condition | 4 mL (2 mL min.) plasma from 1 Lavender Top (EDTA) tube. Separate plasma aseptically within 2 hours of collection. Ship refrigerated or frozen. 12 hours fasting is recommended. |
| Report Delivery | Sample Daily by 11 am; Report 13 days |
| Method | PCR |
| Test type | Disorders of Liver |
| Doctor | Gastroenterologist |
| Test Department: | MOLECULAR DIAGNOSTICS |
| Pre Test Information | 12 hours fasting is recommended. |
| Test Details |
The PCR test is a highly sensitive and specific method used to detect the presence of the hepatitis D virus (HDV) in a person’s blood. PCR stands for polymerase chain reaction, which is a laboratory technique that amplifies specific segments of DNA or RNA. In the case of HDV detection, the PCR test targets and amplifies the viral RNA present in the blood sample. This amplification allows for the detection of even small amounts of the virus, making the PCR test a reliable method for diagnosing hepatitis D. The PCR test for HDV detection involves several steps. First, the viral RNA is extracted from the blood sample. Then, specific primers and probes that are complementary to the viral RNA are added to the sample. These primers and probes bind to the viral RNA if it is present. Next, an enzyme called reverse transcriptase is used to convert the viral RNA into complementary DNA (cDNA). This cDNA is then amplified using a DNA polymerase enzyme, creating multiple copies of the viral DNA. The amplification process is carried out through a series of temperature cycles, which allow the DNA polymerase enzyme to synthesize new DNA strands. Each cycle doubles the amount of DNA, resulting in a significant increase in the amount of viral DNA if the virus is present. Finally, the amplified DNA is analyzed using various methods, such as gel electrophoresis or fluorescent probes, to determine the presence or absence of the hepatitis D virus. Overall, the PCR test for hepatitis D virus detection is a sensitive and specific method that allows for early diagnosis and monitoring of HDV infection. It is a valuable tool in the management of hepatitis D and helps in determining appropriate treatment options. |