ELECTRON MICROSCOPY TEM Test
Components: Price 1640.0 AED
Sample Condition: Submit Kidney / Muscle / Nerve / Other tissue biopsies in 3% Buffered Glutaraldehyde solution available from LPL. Ship refrigerated. Duly filled Electron Microscopy Requisition Form (Form 7) is mandatory.
Report Delivery: Sample Daily by 6 pm; Report 22 days
Method: Transmission Electron microscopy
Test type: Disorders of Kidney, Musculoskeletal disorders, Disorders of Nerves
Doctor: Nephrologist, Neurologist
Test Department: HISTOLOGY
Pre Test Information: Duly filled Electron Microscopy Requisition Form (Form 7) is mandatory.
Test Details:
- Sample Preparation: The specimen needs to be prepared in a thin section, typically less than 100 nanometers thick, to allow the transmission of electrons. This can be achieved through techniques such as ultramicrotomy, ion milling, or focused ion beam (FIB) milling.
- Loading the Sample: The prepared specimen is then loaded onto a TEM grid, which is a thin support film with a mesh structure. The grid is placed onto a sample holder, which is inserted into the TEM instrument.
- Vacuum Chamber: The entire TEM system operates under high vacuum conditions to prevent the scattering and absorption of electrons by air molecules. The vacuum chamber is typically pumped down to a pressure of 10^-5 to 10^-7 pascals.
- Electron Beam: A beam of electrons is generated by an electron gun, which consists of a heated filament that emits electrons. The electrons are accelerated and focused by electromagnetic lenses, forming a narrow beam that passes through the specimen.
- Interaction with the Specimen: As the electron beam passes through the specimen, it interacts with the atoms and electrons within the material. This interaction causes the electrons to scatter, producing a variety of signals.
- Image Formation: The scattered electrons are collected by a detector, such as a fluorescent screen or a charge-coupled device (CCD) camera. The collected signals are then processed to generate an image of the specimen.
- High-Resolution Imaging: TEM is capable of achieving high resolution, down to sub-angstrom levels, allowing the visualization of individual atoms. This is achieved by using a combination of advanced electron optics, aberration correction, and image processing techniques.
- Elemental Analysis: In addition to imaging, TEM can also be used for elemental analysis. By using energy-dispersive X-ray spectroscopy (EDS) or electron energy-loss spectroscopy (EELS), the composition and distribution of elements within the specimen can be determined.
- Data Interpretation: The acquired TEM images and spectra are analyzed and interpreted to gain insights into the structure, composition, and properties of the specimen. This information can be used to study materials at the atomic scale, investigate biological samples, analyze nanomaterials, and much more.
Overall, TEM is a versatile technique that provides detailed information about the internal structure and composition of a wide range of materials. It has revolutionized our understanding of the nanoscale world and continues to be a valuable tool for scientific research and technological advancements.
Test Name | ELECTRON MICROSCOPY TEM Test |
---|---|
Components | |
Price | 1640.0 AED |
Sample Condition | Submit Kidney \/ Muscle \/ Nerve \/ Other tissue biopsies in 3% Buffered Glutaraldehyde solution available from LPL. Ship refrigerated. Duly filled Electron Microscopy Requisition Form (Form 7) is mandatory. |
Report Delivery | Sample Daily by 6 pm; Report 22 days |
Method | Transmission Electron microscopy |
Test type | Disorders of Kidney, Musculoskeletal disorders, Disorders of Nerves |
Doctor | Nephrologist, Neurologist |
Test Department: | HISTOLOGY |
Pre Test Information | Duly filled Electron Microscopy Requisition Form (Form 7) is mandatory. |
Test Details |
Transmission Electron Microscopy (TEM) is a powerful imaging technique that uses a beam of electrons to visualize the internal structure of a specimen at high resolution. It is widely used in various fields of science and technology, including materials science, biology, chemistry, and nanotechnology. The TEM test involves several steps: 1. Sample Preparation: The specimen needs to be prepared in a thin section, typically less than 100 nanometers thick, to allow the transmission of electrons. This can be achieved through techniques such as ultramicrotomy, ion milling, or focused ion beam (FIB) milling. 2. Loading the Sample: The prepared specimen is then loaded onto a TEM grid, which is a thin support film with a mesh structure. The grid is placed onto a sample holder, which is inserted into the TEM instrument. 3. Vacuum Chamber: The entire TEM system operates under high vacuum conditions to prevent the scattering and absorption of electrons by air molecules. The vacuum chamber is typically pumped down to a pressure of 10^-5 to 10^-7 pascals. 4. Electron Beam: A beam of electrons is generated by an electron gun, which consists of a heated filament that emits electrons. The electrons are accelerated and focused by electromagnetic lenses, forming a narrow beam that passes through the specimen. 5. Interaction with the Specimen: As the electron beam passes through the specimen, it interacts with the atoms and electrons within the material. This interaction causes the electrons to scatter, producing a variety of signals. 6. Image Formation: The scattered electrons are collected by a detector, such as a fluorescent screen or a charge-coupled device (CCD) camera. The collected signals are then processed to generate an image of the specimen. 7. High-Resolution Imaging: TEM is capable of achieving high resolution, down to sub-angstrom levels, allowing the visualization of individual atoms. This is achieved by using a combination of advanced electron optics, aberration correction, and image processing techniques. 8. Elemental Analysis: In addition to imaging, TEM can also be used for elemental analysis. By using energy-dispersive X-ray spectroscopy (EDS) or electron energy-loss spectroscopy (EELS), the composition and distribution of elements within the specimen can be determined. 9. Data Interpretation: The acquired TEM images and spectra are analyzed and interpreted to gain insights into the structure, composition, and properties of the specimen. This information can be used to study materials at the atomic scale, investigate biological samples, analyze nanomaterials, and much more. Overall, TEM is a versatile technique that provides detailed information about the internal structure and composition of a wide range of materials. It has revolutionized our understanding of the nanoscale world and continues to be a valuable tool for scientific research and technological advancements. |