It is the process of pairing and ordering all the chromosomes of an organism, thus providing a genome-wide snapshot of an individual’s chromosomes. Karyotyping single is prepared using standardised staining procedures that reveal characteristic structural features for each chromosome. Clinical cytogeneticists analyse human karyotypes to detect gross genetic changes—anomalies involving several megabases or more of DNA.
Reasons to take a Karyotype test:
Your baby or young child may need a test if he or she has signs of a genetic disorder. There are many types of genetic disorders, each with different symptoms. You and your health care provider can talk about whether testing is recommended.
If you are a woman, you may need a karyotype test if you’ve had trouble getting pregnant or have had several miscarriages. While one miscarriage is not uncommon, if you have had several, it may be due to a chromosomal problem.
Peripheral blood for karyotyping(Single):
A chromosomal karyotype is used to detect chromosome abnormalities and thus used to diagnose genetic diseases, some congenital disabilities, and certain disorders of the blood and lymphatic system. Peripheral blood studies best study constitutional chromosomal patterns. Hence this is the most commonly used tissue for cytogenetic investigation. The steps include growing the lymphocytes by stimulation, harvesting of the cultures and staining. In this test, only 1 individual is examined to detect chromosomal abnormalities.