IMMUNOPHENOTYPING BY FLOW CYTOMETRY ZAP-70 Test
Test Cost: AED 480.0
Introduction
The ZAP-70 test is a type of immunophenotyping test performed using flow cytometry. It is primarily used in the diagnosis and prognosis of chronic lymphocytic leukemia (CLL). The test helps in determining the presence or absence of ZAP-70 protein expression on CLL cells, which is associated with a more aggressive form of CLL and a poorer prognosis.
Test Components
- Price: AED 480.0
- Sample Condition:
- 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube
- 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube OR
- 2 mL (1 mL min.) Bone marrow in 1 Green Top (Sodium heparin) tube
- Ship immediately at 18-22°C. DO NOT REFRIGERATE OR FREEZE.
- Specify time, date, and clinical details on the test request form.
Report Delivery
Sample: Daily by 9 am
Report: Same day
Method
Flow Cytometry
Test Type
Cancer
Doctor
Hematologist, Oncologist
Test Department
FLOW CYTOMETRY
Pre Test Information
Give brief clinical history.
Test Details
The ZAP-70 test is performed by collecting a sample of blood or bone marrow from the patient. The cells in the sample are stained with fluorescent antibodies specific to ZAP-70 protein and other markers to identify different cell types. The sample is then analyzed using flow cytometry, a technique that measures the characteristics of individual cells as they pass through a laser beam. The flow cytometer detects the fluorescence emitted by the stained cells and generates data on the expression levels of ZAP-70 protein on CLL cells.
The results of the ZAP-70 test are interpreted by comparing the fluorescence intensity of the ZAP-70 positive cells to a control sample. Based on the results, patients can be classified into ZAP-70 positive or ZAP-70 negative groups. ZAP-70 positive CLL is associated with a more aggressive disease course and may require more aggressive treatment options. On the other hand, ZAP-70 negative CLL is associated with a more indolent disease course and a better prognosis.
In summary, the ZAP-70 test is a flow cytometry-based immunophenotyping test used to determine the expression levels of ZAP-70 protein on CLL cells. It helps in predicting the prognosis and guiding treatment decisions for patients with CLL.
| Test Name | IMMUNOPHENOTYPING BY FLOW CYTOMETRY ZAP-70 Test |
|---|---|
| Components | |
| Price | 480.0 AED |
| Sample Condition | 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tubeAND 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube OR 2 mL (1 mL min.) Bone marrow in 1 Green Top (Sodium heparin) tube. Ship immediately at 18\u0192??22?\u00f8C. DO NOT REFRIGERATE OR FREEZE. Specify time, date and clinical details on test request form. |
| Report Delivery | Sample Daily by 9 am;Report Same day |
| Method | Flow Cytometry |
| Test type | Cancer |
| Doctor | Hematologist, Oncologist |
| Test Department: | FLOW CYTOMETRY |
| Pre Test Information | Give brief clinical history. |
| Test Details |
The ZAP-70 test is a type of immunophenotyping test performed using flow cytometry. ZAP-70 stands for zeta-chain-associated protein kinase 70, which is a protein involved in the signaling pathway of T-cells. The test is primarily used in the diagnosis and prognosis of chronic lymphocytic leukemia (CLL). It helps in determining the presence or absence of ZAP-70 protein expression on CLL cells. ZAP-70 expression is associated with a more aggressive form of CLL and a poorer prognosis. During the test, a sample of blood or bone marrow is collected from the patient. The cells in the sample are stained with fluorescent antibodies specific to ZAP-70 protein and other markers to identify different cell types. The sample is then analyzed using flow cytometry, a technique that measures the characteristics of individual cells as they pass through a laser beam. The flow cytometer detects the fluorescence emitted by the stained cells and generates data on the expression levels of ZAP-70 protein on CLL cells. The results are interpreted by comparing the fluorescence intensity of the ZAP-70 positive cells to a control sample. Based on the results, patients can be classified into ZAP-70 positive or ZAP-70 negative groups. ZAP-70 positive CLL is associated with a more aggressive disease course and may require more aggressive treatment options. On the other hand, ZAP-70 negative CLL is associated with a more indolent disease course and a better prognosis. In summary, the ZAP-70 test is a flow cytometry-based immunophenotyping test used to determine the expression levels of ZAP-70 protein on CLL cells. It helps in predicting the prognosis and guiding treatment decisions for patients with CLL. |
