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Immunohistochemistry SALL 4 Test Cost

Original price was: 550 د.إ.Current price is: 500 د.إ.

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Immunohistochemistry (IHC) SALL4 Test is a specialized diagnostic procedure utilized to detect the presence of the SALL4 protein in tissue samples. SALL4 is a transcription factor critical for embryonic development and has been identified as a marker in various types of cancers, including germ cell tumors and some leukemias. The test involves the application of specific antibodies against the SALL4 protein on tissue sections, which are then visualized under a microscope. This method allows pathologists to assess the expression and distribution of SALL4, aiding in the diagnosis, prognostication, and sometimes in the therapeutic decision-making process for cancer patients.

At DNA Labs UAE, the Immunohistochemistry SALL4 Test is offered for a cost of 500 AED. The laboratory is equipped with state-of-the-art facilities and experienced professionals to ensure accurate and reliable test results. This test is an essential tool in the armamentarium for oncologists and pathologists, providing valuable insights into the molecular characteristics of tumors, which is pivotal for personalized medicine approaches in cancer treatment.

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IMMUNOHISTOCHEMISTRY SALL 4 Test

Test Name: IMMUNOHISTOCHEMISTRY SALL 4 Test

Components: Price: 500.0 AED

Sample Condition: Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Report Delivery: Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex: 7 days

Method: Immunohistochemistry

Test type: Cancer

Doctor: Oncologist, Pathologist

Test Department: HISTOLOGY

Pre Test Information: Provide a copy of the Histopathology report, Site of biopsy and Clinical history.

Test Details

SALL4 is a transcription factor that plays a critical role in embryonic development and stem cell pluripotency. It is also expressed in certain types of cancer, including germ cell tumors, hepatocellular carcinoma, and acute myeloid leukemia.

Immunohistochemistry (IHC) is a commonly used technique to detect and localize specific proteins in tissue samples. To perform an immunohistochemistry test for SALL4, the following steps can be followed:

  1. Tissue Preparation: Obtain a tissue sample from the patient or use a pre-existing tissue section. Fix the tissue in formalin and embed it in paraffin wax. Cut thin sections (usually around 4-5 μm) using a microtome.
  2. Deparaffinization and Rehydration: Place the tissue sections on glass slides and deparaffinize them by immersing the slides in xylene or other suitable clearing agents. Then, rehydrate the sections by passing them through a series of graded alcohols (e.g., 100%, 95%, 70%, and 50% ethanol).
  3. Antigen Retrieval: Perform antigen retrieval to unmask the target antigen. This can be done by heat-induced epitope retrieval (HIER) using a microwave or pressure cooker, or by enzymatic digestion. Different antigen retrieval methods can be optimized for SALL4 detection.
  4. Blocking: Block non-specific binding sites on the tissue sections by incubating them with a blocking solution, typically containing serum or bovine serum albumin (BSA). This helps to reduce background staining.
  5. Primary Antibody Incubation: Incubate the tissue sections with a primary antibody against SALL4. The primary antibody should be specific to SALL4 and can be either monoclonal or polyclonal. Incubation time and temperature may vary depending on the antibody used, but overnight incubation at 4°C is commonly recommended.
  6. Secondary Antibody Incubation: Wash the tissue sections to remove unbound primary antibody and incubate them with a secondary antibody conjugated to a detection system, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). The secondary antibody should be specific to the host species of the primary antibody.
  7. Visualization: Apply a chromogenic substrate to the tissue sections that reacts with the detection system of the secondary antibody. This will produce a visible color reaction at the site of SALL4 expression. The choice of chromogenic substrate depends on the detection system used (e.g., DAB for HRP).
  8. Counterstaining: Counterstain the tissue sections with a suitable dye, such as hematoxylin, to visualize the overall tissue morphology. This helps in locating the specific SALL4-positive cells within the tissue.
  9. Mounting: Dehydrate the tissue sections through a series of graded alcohols and xylene, and then mount a coverslip using a suitable mounting medium.
  10. Microscopic Examination: Finally, examine the stained tissue sections under a microscope to assess the expression and localization of SALL4. Positive staining will appear as brown coloration in the nucleus or cytoplasm of the target cells.

It is important to note that the specific protocols and reagents used for SALL4 immunohistochemistry may vary depending on the laboratory and the specific antibody being used. Optimization of the protocol may be necessary for different tissue types or experimental conditions.

Test Name IMMUNOHISTOCHEMISTRY SALL 4 Test
Components
Price 500.0 AED
Sample Condition Submit tumor tissue in 10% Formal-saline OR Formalin fixed paraffin embedded block. Ship at room temperature. Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Report Delivery Sample Daily by 6 pm; Report Block: 5 days Tissue Biopsy: 5 days Tissue large complex : 7 days
Method Imunohistochemistry
Test type Cancer
Doctor Oncologist, Pathologist
Test Department: HISTOLOGY
Pre Test Information Provide a copy of the Histopathology report, Site of biopsy and Clinical history.
Test Details

SALL4 is a transcription factor that plays a critical role in embryonic development and stem cell pluripotency. It is also expressed in certain types of cancer, including germ cell tumors, hepatocellular carcinoma, and acute myeloid leukemia. Immunohistochemistry (IHC) is a commonly used technique to detect and localize specific proteins in tissue samples.

To perform an immunohistochemistry test for SALL4, the following steps can be followed:

1. Tissue Preparation: Obtain a tissue sample from the patient or use a pre-existing tissue section. Fix the tissue in formalin and embed it in paraffin wax. Cut thin sections (usually around 4-5 m) using a microtome.

2. Deparaffinization and Rehydration: Place the tissue sections on glass slides and deparaffinize them by immersing the slides in xylene or other suitable clearing agents. Then, rehydrate the sections by passing them through a series of graded alcohols (e.g., 100%, 95%, 70%, and 50% ethanol).

3. Antigen Retrieval: Perform antigen retrieval to unmask the target antigen. This can be done by heat-induced epitope retrieval (HIER) using a microwave or pressure cooker, or by enzymatic digestion. Different antigen retrieval methods can be optimized for SALL4 detection.

4. Blocking: Block non-specific binding sites on the tissue sections by incubating them with a blocking solution, typically containing serum or bovine serum albumin (BSA). This helps to reduce background staining.

5. Primary Antibody Incubation: Incubate the tissue sections with a primary antibody against SALL4. The primary antibody should be specific to SALL4 and can be either monoclonal or polyclonal. Incubation time and temperature may vary depending on the antibody used, but overnight incubation at 4C is commonly recommended.

6. Secondary Antibody Incubation: Wash the tissue sections to remove unbound primary antibody and incubate them with a secondary antibody conjugated to a detection system, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). The secondary antibody should be specific to the host species of the primary antibody.

7. Visualization: Apply a chromogenic substrate to the tissue sections that reacts with the detection system of the secondary antibody. This will produce a visible color reaction at the site of SALL4 expression. The choice of chromogenic substrate depends on the detection system used (e.g., DAB for HRP).

8. Counterstaining: Counterstain the tissue sections with a suitable dye, such as hematoxylin, to visualize the overall tissue morphology. This helps in locating the specific SALL4-positive cells within the tissue.

9. Mounting: Dehydrate the tissue sections through a series of graded alcohols and xylene, and then mount a coverslip using a suitable mounting medium.

10. Microscopic Examination: Finally, examine the stained tissue sections under a microscope to assess the expression and localization of SALL4. Positive staining will appear as brown coloration in the nucleus or cytoplasm of the target cells.

It is important to note that the specific protocols and reagents used for SALL4 immunohistochemistry may vary depending on the laboratory and the specific antibody being used. Optimization of the protocol may be necessary for different tissue types or experimental conditions.

SKU: TEST-6310 Category:

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